State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, China.
Genome. 2011 Aug;54(8):611-9. doi: 10.1139/g11-022. Epub 2011 Jul 27.
Rice stripe disease, caused by Rice stripe virus (RSV) and transmitted by the small brown planthopper (Laodelphax striatellus Fallen), is one of the most serious viral diseases of rice in temperate East Asian production regions. Prior quantitative trait loci (QTL) mapping has established that Oryza sativa L. subsp. indica 'IR24' carries positive alleles at the three loci qSTV3, qSTV7, and qSTV11-i. Here, we report an advanced backcross analysis based on three selected chromosome segment substitution lines (CSSLs), each predicted to carry one of these three QTL. Three sets of BC(4)F(2:3) populations were bred from a cross between the critical CSSL and its recurrent parent Oryza sativa L. subsp. japonica 'Asominori'. Both qSTV3 and qSTV11-i were detected in their respective population, but qSTV7 was not. An allelic analysis based on a known carrier of the major RSV resistance gene Stvb-i, which is located on chromosome 11, showed that qSTV11-i was not allelic with Stvb-i. A large mapping population was used to delimit the location of qSTV11-i to a 73.6-kb region. The de novo markers developed for this purpose will be useful as marker-assisted selection tools in efforts to introduce qSTV11-i into breeding programmes aiming to improve the level of RSV resistance.
水稻条纹病毒病由水稻条纹病毒（RSV）引起，通过褐飞虱（Laodelphax striatellus Fallen）传播，是东亚温带稻区最严重的病毒性病害之一。先前的数量性状位点（QTL）定位研究表明，籼稻品种‘IR24’在三个位点 qSTV3、qSTV7 和 qSTV11-i 上携带正等位基因。在这里，我们报告了一项基于三个选定的染色体片段代换系（CSSLs）的高级回交分析，每个 CSSL 都被预测携带其中一个 QTL。从关键 CSSL 与其轮回亲本籼稻品种‘Asominori’之间的杂交中，培育了三组 BC(4)F(2:3)群体。在各自的群体中检测到 qSTV3 和 qSTV11-i，但未检测到 qSTV7。基于位于第 11 号染色体上 RSV 主要抗性基因 Stvb-i 的已知携带者的等位基因分析表明，qSTV11-i 与 Stvb-i 不同。利用一个大的作图群体，将 qSTV11-i 的位置限定在一个 73.6-kb 的区域内。为此目的开发的从头标记将成为引入 qSTV11-i 到旨在提高 RSV 抗性水平的育种计划中的有用的标记辅助选择工具。
