Fujino Y, Kawatsu R, Inagaki F, Umeda A, Yokoyama T, Okaue Y, Iwai S, Ogata S, Ohshima T, Doi K
Department of Chemistry and Physics of Condensed Matter, Faculty of Science, Kyushu University, Japan.
J Appl Microbiol. 2008 Jan;104(1):70-8. doi: 10.1111/j.1365-2672.2007.03528.x. Epub 2007 Sep 10.
To identify an extreme thermophile, strain TMY, isolated from silica scale from the geothermal electric power plant and to examine microdiversity of Thermus thermophilus strains.
The isolated strain TMY was identified by morphological, biochemical and physiological tests. Phylogenetic comparison of the strain and other Thermus strains with 16S rDNA analysis, RAPD and ERIC-PCR fingerprinting were performed. Strain TMY was closely related to strain which was isolated from a hot spring in New Zealand and shown to belong to the Japanese Thermus cluster. However, there were considerable genetic differences between strain TMY and other Thermus species using DNA fingerprinting.
Based on morphological, physiological and genetic properties, strain TMY could be a strain of T. thermophilus. The distinct properties of strain TMY suggest that microdiversity of T. thermophilus strains should be considered.
The results of this study have demonstrated genetic diversity within T. thermophilus strains, which were previously masked by an almost identical 16S rDNA sequence. RAPD and ERIC-PCR could be potential methods for distinguishing between Thermus strains.
鉴定从地热发电厂硅垢中分离出的嗜热极端菌TMY菌株，并研究嗜热栖热菌菌株的微观多样性。
通过形态学、生化和生理学试验对分离出的TMY菌株进行鉴定。利用16S rDNA分析、随机扩增多态性DNA（RAPD）和肠杆菌基因间重复共有序列聚合酶链反应（ERIC-PCR）指纹图谱对该菌株与其他嗜热栖热菌菌株进行系统发育比较。TMY菌株与从新西兰温泉中分离出的、显示属于日本嗜热栖热菌群的菌株密切相关。然而，使用DNA指纹图谱分析发现，TMY菌株与其他嗜热栖热菌物种之间存在相当大的遗传差异。
基于形态学、生理学和遗传学特性，TMY菌株可能是嗜热栖热菌的一个菌株。TMY菌株的独特特性表明应考虑嗜热栖热菌菌株的微观多样性。
本研究结果证明了嗜热栖热菌菌株内部的遗传多样性，此前这种多样性被几乎相同的16S rDNA序列所掩盖。RAPD和ERIC-PCR可能是区分嗜热栖热菌菌株的潜在方法。
