Bakopoulou Athina, Tsiftsoglou Asterios, Galaktidou Grammati, Markala Dimitra, Triviai Ioanna, Garefis Pavlos
Department of Fixed & Implant Prosthodontics, School of Dentistry, Aristotle University of Thessaloniki, Greece.
Eur J Oral Sci. 2007 Oct;115(5):397-407. doi: 10.1111/j.1600-0722.2007.00478.x.
Previous studies have shown that in vitro exposure to single compounds released from composite resins may induce cell death. In the present study the effects of eluates from commercially available composite resins used for direct or indirect restorations were evaluated on the cell cycle progression and type of cell death of cultured WEHI 13 var fibroblasts. Cells exposed to eluates of the materials were assessed for cytotoxicity by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for cell death, for cell cycle profiles by flow cytometry, for caspase-3 biochemically and by immunocytochemistry, and for morphological changes by fluorescence microscopy with acridine orange. The direct composite resin eluates induced extensive apoptosis, followed by secondary necrosis. This was accompanied by cell enlargement, micromultinucleation, chromatin disintegration, cell cycle arrest at different phases, and caspase-3 activation. The composites for indirect restorations were much less cytotoxic at all biological end-points investigated. The findings suggest that composite resins used for direct and indirect dental restorations differ in their cytotoxic potential and their ability to affect basic cellular functions. This underlines the impact of improved polymerization with respect to their biologic behavior.
先前的研究表明，体外暴露于复合树脂释放的单一化合物可能会诱导细胞死亡。在本研究中，评估了用于直接或间接修复的市售复合树脂洗脱液对培养的WEHI 13 var成纤维细胞的细胞周期进程和细胞死亡类型的影响。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐(MTT)法检测细胞死亡情况来评估暴露于材料洗脱液中的细胞的细胞毒性，通过流式细胞术检测细胞周期谱，通过生化方法和免疫细胞化学检测半胱天冬酶-3，通过吖啶橙荧光显微镜检测形态变化。直接复合树脂洗脱液诱导广泛的细胞凋亡，随后是继发性坏死。这伴随着细胞肿大、微多核化、染色质解体、细胞周期在不同阶段停滞以及半胱天冬酶-3激活。在所有研究的生物学终点上，间接修复用的复合材料细胞毒性要小得多。研究结果表明，用于直接和间接牙齿修复的复合树脂在细胞毒性潜力和影响基本细胞功能的能力方面存在差异。这强调了改进聚合对其生物学行为的影响。
