Lai Y-L, Chen Y-T, Lee S-Y, Shieh T-M, Hung S-L
Dental Department, Veterans General Hospital-Taipei, Taipei, Taiwan.
J Oral Rehabil. 2004 Dec;31(12):1165-72. doi: 10.1111/j.1365-2842.2004.01355.x.
Cytotoxic effects of resin liquids of three in situ relining dental polymers, Alike, Kooliner, and Tokuso Rebase, and their major components, methyl methacrylate (MMA), isobutyl methacrylate (IBMA), and 1,6-hexanediol dimethacrylate (1,6-HDMA) were investigated. The concentrations of major monomers in these resin liquids were determined by high-performance liquid chromatography. Cellular viability of human gingival fibroblasts (GF) and periodontal ligament (PDL) cells were evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide assay. Moreover, patterns of cell death were analysed using annexin V/propidium iodide staining with flow cytometry. The results indicated that Alike liquid contained 91.3% MMA, Kooliner liquid contained 94.5% IBMA, and Tokuso Rebase liquid contained 65.8% 1,6-HDMA. All materials examined had cytotoxic effects on GF and PDL cells in dose-dependent manners. Tokuso Rebase liquid appeared to be the most cytotoxic among the various resin liquids examined. The effects of Kooliner and Tokuso Rebase liquids may have resulted from IBMA and 1,6-HDMA, respectively. Furthermore, the majority of treated cells died from necrosis; whereas a small portion of cells died from apoptosis. In conclusion, the results demonstrated that these liquid forms of dental polymers and their major monomers cause cytotoxic reactions. The direct relining procedure that cures these materials in situ should be used cautiously.
研究了三种用于口腔原位重衬的牙科聚合物（Alike、Kooliner和Tokuso Rebase）的树脂液及其主要成分甲基丙烯酸甲酯（MMA）、甲基丙烯酸异丁酯（IBMA）和1,6 - 己二醇二甲基丙烯酸酯（1,6 - HDMA）的细胞毒性作用。通过高效液相色谱法测定这些树脂液中主要单体的浓度。采用3 - [4,5 - 二甲基噻唑 - 2 - 基] - 2,5 - 二苯基四氮唑溴盐法评估人牙龈成纤维细胞（GF）和牙周膜（PDL）细胞的细胞活力。此外，使用膜联蛋白V/碘化丙啶染色结合流式细胞术分析细胞死亡模式。结果表明，Alike液含有91.3%的MMA，Kooliner液含有94.5%的IBMA，Tokuso Rebase液含有65.8%的1,6 - HDMA。所有检测材料对GF和PDL细胞均有剂量依赖性的细胞毒性作用。在所有检测的树脂液中，Tokuso Rebase液的细胞毒性似乎最大。Kooliner液和Tokuso Rebase液的作用可能分别由IBMA和1,6 - HDMA引起。此外，大多数处理过的细胞死于坏死；而一小部分细胞死于凋亡。总之，结果表明这些牙科聚合物的液体形式及其主要单体可引起细胞毒性反应。应谨慎使用在原位固化这些材料的直接重衬程序。
