Wang Jia, Li Jie, Zhuang Hui, Liu She-lan, Li Rong-cheng, Li Yan-ping, Liang Zheng-lun
Department of Microbiology, Peking University Health Science Center, Beijing 100083, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 Apr;28(4):331-3.
To investigate the association of heptitis B virus (HBV) genotypes and precore(PreC)/basal core promoter(BCP) mutation with interruption failure of HBV vaccination in mother-to-infant transmission.
A total number of 208 serum samples were collected from infants and mothers,including 16 infants who had become HBsAg-positive despite a complete and timely course of immunization and another 88 infants successfully protected from mother-to infant HBV transmission. HBV genotypes were determined by type-specific primers PCR method. PreC/BCP mutations were detected by direct sequencing of PCR products, and Clustal W 1.8 software was applied to analyzing the sequences.
Of 16 mothers who were having vaccine failure infants, 15 (93.8%) were HBeAg positive and infected with genotype C (15/15, 100%). Among 88 mothers of having children being protected by vaccine, 51 (58.0%) were HBeAg positive, with 45.1% (23/51) of genotype C. The proportion of genotype C in HBeAg mothers of infants with vaccine failure, was significantly higher than that of mothers with vaccine protected infants (chi2 = 14.3, P = 0.003). However, the frequencies of T1762/A1764 mutations had no significant differences between genotype C HBeAg positive mothers with vaccine failure or protected infants (33.3% and 13.3%, respectively, P = 0.4). No A1896 mutation was found in these two groups.
HBV genotype C might contribute to the immune failure of HBV vaccination in mother-to-infant transmission, while PreC/BCP mutation might not have correlation with it.
探讨乙型肝炎病毒（HBV）基因型及前C区/基本核心启动子（BCP）突变与母婴传播中HBV疫苗接种阻断失败的相关性。
收集208例婴儿及其母亲的血清样本，其中包括16例尽管全程及时接种疫苗仍出现HBsAg阳性的婴儿，以及另外88例成功阻断母婴HBV传播的婴儿。采用型特异性引物PCR法确定HBV基因型。通过PCR产物直接测序检测前C区/BCP突变，并应用Clustal W 1.8软件分析序列。
在16例疫苗接种失败婴儿的母亲中，15例（93.8%）HBeAg阳性且感染C基因型（15/15，100%）。在88例其子女获得疫苗保护的母亲中，51例（58.0%）HBeAg阳性，其中C基因型占45.1%（23/51）。疫苗接种失败婴儿的母亲中C基因型在HBeAg阳性母亲中的比例显著高于其子女获得疫苗保护的母亲（χ2 = 14.3，P = 0.003）。然而，C基因型HBeAg阳性的疫苗接种失败或成功阻断母婴传播的母亲中T1762/A1764突变频率无显著差异（分别为33.3%和13.3%，P = 0.4）。两组均未发现A1896突变。
HBV C基因型可能导致母婴传播中HBV疫苗免疫失败，而前C区/BCP突变可能与之无关。
