Sebastian K S, Thampan Raghava Varman
Division of Molecular Endocrinology, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala, India.
Chem Biol Interact. 2007 Nov 20;170(2):135-43. doi: 10.1016/j.cbi.2007.07.011. Epub 2007 Jul 28.
The effect of aqueous and ethanol extracts of soybean and fenugreek on the growth of MCF-7 cells, an estrogen receptor positive breast cancer cell line, has been examined in this study. Soybean is well known for the presence of phytoestrogens and fenugreek is reported to have medicinal use including anticancer properties. In a dose dependent manner soybean aqueous and ethanol extract promoted the growth and DNA synthesis in MCF-7 cells. On the contrary ethanol extract of fenugreek decreased the cell viability and induced early apoptotic changes such as flipping of phosphatidylserine and decrease of mitochondrial membrane potential. Degradation of cellular DNA into fragments comprising multiples of approximately 180-200 base pair was also observed. Cell cycle analysis by flow cytometry showed the presence of a subG1 apoptotic population which was more prominent at higher concentrations along with cell cycle arrest at G2/M phase. Our experiments show that while the soybean extract acts as a promoter of MCF-7 cell growth, the fenugreek extract induces apoptosis.
本研究检测了大豆和胡芦巴的水提取物及乙醇提取物对雌激素受体阳性乳腺癌细胞系MCF-7细胞生长的影响。大豆因含有植物雌激素而闻名，据报道胡芦巴具有药用价值，包括抗癌特性。大豆水提取物和乙醇提取物以剂量依赖的方式促进MCF-7细胞的生长和DNA合成。相反，胡芦巴乙醇提取物降低了细胞活力，并诱导了早期凋亡变化，如磷脂酰丝氨酸外翻和线粒体膜电位降低。还观察到细胞DNA降解为约180 - 200碱基对倍数的片段。通过流式细胞术进行的细胞周期分析显示存在一个亚G1期凋亡群体，该群体在较高浓度下更为突出，同时细胞周期在G2/M期停滞。我们的实验表明，大豆提取物可促进MCF-7细胞生长，而胡芦巴提取物则诱导细胞凋亡。
