Guo Liang, Li Xiao-Ming, Bo Xie, Yang Qi, Zeng Guang-Ming, Liao De-xiang, Liu Jing-Jin
College of Environmental Science and Engineering, Hunan University, Hunan, Changsha 410082, China.
Bioresour Technol. 2008 Jun;99(9):3651-8. doi: 10.1016/j.biortech.2007.07.026. Epub 2007 Sep 11.
Hydrogen production by sterilization, microwave and ultrasonication pretreated waste sludge was investigated in this study. A new strain of Pseudomonas sp. GZ1 (EF551040) was inoculated in pretreated waste sludge to produce hydrogen. The experimental results showed that different pretreated sludge had evident differences in the yield of hydrogen production and lag time. Sterilized sludge had the largest yield of hydrogen production, and the maximum yield was 15.02 ml/gTCOD. The lag time of using sterilized sludge was 15 h, longer than other two pretreated sludge. Using the ultrasonicated sludge, the hydrogen production yield was smallest and lag time was shortest in the three pretreated sludge. Protein and carbohydrate could be released from waste sludge by pretreatment. Protein was the main nutrient used for hydrogen production. The concentration of protein, carbohydrate and SCOD increased after pretreatment and fermentation. The impacts of different pretreatments on hydrogen production were also discussed in detail.
本研究考察了经过灭菌、微波和超声预处理的剩余污泥产氢情况。将一株新的假单胞菌属菌株GZ1（EF551040）接种到预处理后的剩余污泥中以产氢。实验结果表明，不同预处理的污泥在产氢量和滞后期存在明显差异。灭菌污泥的产氢量最大，最大产氢量为15.02 ml/gTCOD。使用灭菌污泥的滞后期为15小时，比其他两种预处理污泥更长。在三种预处理污泥中，使用超声处理的污泥产氢量最小且滞后期最短。预处理可使剩余污泥中的蛋白质和碳水化合物释放出来。蛋白质是产氢的主要营养物质。预处理和发酵后，蛋白质、碳水化合物和溶解性化学需氧量（SCOD）的浓度增加。同时还详细讨论了不同预处理对产氢的影响。
