Environmental Technology Unit, Department Environmental Science, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Appl Biochem Biotechnol. 2012 Jan;166(2):389-401. doi: 10.1007/s12010-011-9434-5. Epub 2011 Nov 19.
Dark anaerobic fermentation is an interesting alternative method for producing biohydrogen (H(2)) as a renewable fuel because of its low cost and various usable organic substrates. Pulping sludge from wastewater treatment containing plentiful cellulosic substrate could be feasibly utilized for H(2) production by dark fermentation. The objective of this study was to investigate the optimal proportion of pulping sludge to paper waste, the optimal initial pH, and the optimal ratio of carbon and nitrogen (C/N) for H(2) production by anaerobic seed sludge pretreated with heat. The pulping sludge was pretreated with NaOH solution at high temperature and further hydrolyzed with crude cellulase. Pretreatment of the pulping sludge with 3% NaOH solution under autoclave at 121 °C for 2 h, hydrolysis with 5 FPU crude cellulase at 50 °C, and pH 4.8 for 24 h provided the highest reducing sugar production yield (229.68 ± 2.09 mg/g(TVS)). An initial pH of 6 and a C/N ratio of 40 were optimal conditions for H(2) production. Moreover, the supplement of paper waste in the pulping sludge enhanced the cumulative H(2) production yield. The continuous hydrogen production was further conducted in a glass reactor with nylon pieces as supporting media and the maximum hydrogen production yield was 151.70 ml/g(TVS).
暗发酵是一种很有前途的生产生物氢气（H2）的可再生能源方法，因为它成本低，可利用的有机基质种类多。造纸厂污水中的制浆污泥富含纤维素基质，可通过暗发酵有效地用于生产氢气。本研究的目的是探讨用热预处理的厌氧种子污泥进行暗发酵生产氢气时，制浆污泥与废纸的最佳比例、最佳初始 pH 值和最佳碳氮比（C/N）。制浆污泥先用 3%的 NaOH 溶液在 121°C 的高压釜中预处理 2 小时，然后用 5 FPU 的粗纤维素酶在 50°C、pH4.8 下水解 24 小时，可获得最高的还原糖产率（229.68±2.09mg/g（TVS））。初始 pH 值为 6 和 C/N 比为 40 是最佳的产氢条件。此外，在制浆污泥中添加废纸可提高累积氢气产量。进一步在玻璃反应器中进行连续氢气生产，以尼龙片作为支撑介质，最大氢气产量为 151.70ml/g（TVS）。
