Hsiao Hsien-Yi, Cheng Tzong-Jih, Yang Ge-Ming, Huang I-Jen, Chen Richie L C
Department of Bio-Industrial Mechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan.
Phytochem Anal. 2008 Mar-Apr;19(2):136-40. doi: 10.1002/pca.1026.
A rapid capillary electrophoresis procedure was developed for determining the anti-cancer components, camptothecins, in Nothapodytes foetida. The hydrophobic compound was extracted from plant tissue (ca. 1 mL of DMSO for 100 mg of dried plant tissue) with a water-miscible organic solvent, DMSO, at elevated temperature (60 degrees C). The extract was directly injected into the separation capillary (untreated fused silica, 34 cm in length, 75 microm i.d.) and analysed in MEKC mode (369 nm). Within 5 min of migration, camptothecins were successfully separated and quantified by adding organic modifiers to the running buffer (20% DMSO, 90 mm SDS in 10 mm borate buffer, pH 8.60). The linear dynamic range for camptothecin was from 5 to 400 microg/mL. This method was proven to be very suitable for monitoring the amount of camptothecins during the cultivation of the medicinal plant.
开发了一种快速毛细管电泳方法，用于测定臭椿中抗癌成分喜树碱。采用与水混溶的有机溶剂二甲基亚砜（DMSO）在高温（60℃）下从植物组织中提取该疏水性化合物（100mg干燥植物组织约需1mL DMSO）。提取物直接注入分离毛细管（未处理的熔融石英，长34cm，内径75μm），并在MEKC模式（369nm）下进行分析。在迁移5分钟内，通过向运行缓冲液（20%DMSO，10mM硼酸盐缓冲液pH 8.60中含90mM SDS）中添加有机改性剂，成功分离并定量了喜树碱。喜树碱的线性动态范围为5至400μg/mL。该方法被证明非常适合监测药用植物栽培过程中喜树碱的含量。
