Chen W, Liu L, Li X, Li J, Ji S, Zhang G, Chai Y
School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China.
Biomed Chromatogr. 2000 Dec;14(8):541-3. doi: 10.1002/1099-0801(200012)14:8<541::AID-BMC49>3.0.CO;2-R.
A capillary zone electrophoresis method was developed for the separation and determination of strychnine and brucine in Strychnos nux-vomica L. and its preparation. The factors that could affect the separation were studied, such as the types and concentrations of electrolytes, pH, ionic strength and organic modifier. The optimum running buffer was 20 mmol/L of ammonium acetate containing 0.2 mol/L of glacial acetic acid (pH 3.64). The applied voltage was 25 kV and the wavelength of the UV detector was set at 214 nm. The established method with dopamine hydrochloride as internal standard was linear in the range of 5-100 microg/mL for both strychnine and brucine. The recovery was 102.96% for strychnine and 98.56% for brucine. The extracts of Strychnos nux-vomica and its preparation could be directly injected for analysis.
建立了一种毛细管区带电泳法，用于分离和测定马钱子及其制剂中士的宁和马钱子碱。研究了电解质类型和浓度、pH值、离子强度和有机改性剂等影响分离的因素。最佳运行缓冲液为含0.2 mol/L冰醋酸的20 mmol/L醋酸铵（pH 3.64）。施加电压为25 kV，紫外检测器波长设定为214 nm。以盐酸多巴胺为内标建立的方法，士的宁和马钱子碱在5-100 μg/mL范围内呈线性。士的宁回收率为102.96%，马钱子碱回收率为98.56%。马钱子及其制剂的提取物可直接进样分析。
