Mauri P, Catalano G, Gardana C, Pietta P
Istituto Technologie Biomediche Avanzate, Segrate, Milano, Italy.
Electrophoresis. 1996 Feb;17(2):367-71. doi: 10.1002/elps.1150170213.
The determination of diterpene glycosides from Stevia rebaudiana leaves using capillary electrophoresis is described. Analyses were performed on fused silica capillaries with 20 mM sodium tetraborate buffer, pH 8.3, and 30 mM sodium dodecyl sulfate. The effect of the organic solvent injected with the sample solution on the electrophoretic solution has been confirmed, and an absolute amount of 1.6 nL per injected sample was optimal. Rebaudioside A and steviolbioside were isolated by semipreparative high performance liquid chromatography (HPLC), and their structure was assessed by mass spectrometry.
描述了使用毛细管电泳法测定甜叶菊叶片中的二萜糖苷。分析在熔融石英毛细管上进行，使用pH 8.3的20 mM硼酸钠缓冲液和30 mM十二烷基硫酸钠。已证实注入样品溶液中的有机溶剂对电泳溶液的影响，每个注入样品1.6 nL的绝对量是最佳的。莱鲍迪苷A和甜菊双糖苷通过半制备高效液相色谱（HPLC）分离，并通过质谱法评估其结构。
