Cheng Zhenyu, Woody Owen Z, Song Jiming, Glick Bernard R, McConkey Brendan J
Department of Biology, University of Waterloo, Waterloo, ON, Canada.
Proteomics. 2009 Sep;9(17):4271-4. doi: 10.1002/pmic.200900142.
A proteome reference map containing 326 2-D gel spots representing 275 different proteins was constructed for the plant growth-promoting bacterium Pseudomonas putida UW4. Protein identifications were obtained using Q-TOF MS/MS spectra matching to homologous proteins from other Pseudomonas strains and confirmed by PMF analysis. This data set is accessible at http://world-2dpage.expasy.org/repository/ and will aid in further characterization of Pseudomonas strains and interactions of plant growth-promoting bacterium with the plant rhizosphere environment.
构建了包含326个二维凝胶斑点、代表275种不同蛋白质的蛋白质组参考图谱，用于促进植物生长的恶臭假单胞菌UW4。通过将Q-TOF MS/MS光谱与其他假单胞菌菌株的同源蛋白质进行匹配获得蛋白质鉴定结果，并通过PMF分析进行确认。该数据集可在http://world-2dpage.expasy.org/repository/获取，将有助于进一步表征假单胞菌菌株以及促进植物生长的细菌与植物根际环境的相互作用。
