Chatterjee Souvik, Ghosh Kausik, Raychoudhuri Amit, Pan Arpita, Bhattacharya Mihir Kumar, Mukhopadhyay Asish Kumar, Ramamurthy T, Bhattacharya Sujit Kumar, Nandy Ranjan Kumar
National Institute of Cholera and Enteric Diseases, P-33, C. I. T. Road, Scheme XM, Beliaghata, Kolkata 700 010, India.
J Med Microbiol. 2007 Jun;56(Pt 6):824-832. doi: 10.1099/jmm.0.46982-0.
During 2003, Vibrio cholerae O1 Ogawa was the predominant serotype among diarrhoeal patients admitted to different hospitals in India. With the exception of 3 strains from Kolkata, none of 172 strains examined exhibited resistance to tetracycline, but 45.7 % showed reduced susceptibility to ciprofloxacin. Extensive molecular characterization using randomly amplified polymorphic DNA analysis, ribotyping and PFGE revealed that almost all the strains within a serogroup were clonally related. Along with the H pulsotype, a newly described L pulsotype of recently emerged O1 Inaba strains was detected among the O1 Ogawa strains from 2003. The striking similarity in their molecular properties and antibiograms indicated that at least certain clones of recently emerged Inaba strains from 2004 may have evolved from O1 Ogawa strains. This view was further supported by the detection of a nearly identical wbeT region among the O1 Ogawa and recently emerged Inaba strains, the latter differing only by a single point mutation. Since 2003, a hiatus in the isolation of serogroup O139 was observed and these strains share the same PFGE profiles as those isolated during 2000. Organization of tandemly arranged CTX(El), CTX(Cal) and truncated CTX(Cal) (devoid of ctxAB) prophages was unique among the majority of these O139 strains.
2003年期间，霍乱弧菌O1小川型是印度不同医院收治的腹泻患者中的主要血清型。除了来自加尔各答的3株菌株外，所检测的172株菌株中没有一株对四环素耐药，但45.7%的菌株对环丙沙星的敏感性降低。使用随机扩增多态性DNA分析、核糖体分型和脉冲场凝胶电泳进行的广泛分子特征分析表明，一个血清群内的几乎所有菌株都具有克隆相关性。在2003年的O1小川型菌株中，除了H脉冲型外，还检测到一种新描述的、最近出现的O1稻叶型菌株的L脉冲型。它们分子特性和抗菌谱的显著相似性表明，2004年最近出现的稻叶型菌株的至少某些克隆可能是从O1小川型菌株进化而来的。O1小川型和最近出现的稻叶型菌株中检测到几乎相同的wbeT区域，后者仅存在一个单点突变，这进一步支持了这一观点。自2003年以来，观察到O139血清群分离出现间断，这些菌株与2000年分离的菌株具有相同的脉冲场凝胶电泳图谱。在这些O139菌株中的大多数中，串联排列的CTX(El)、CTX(Cal)和截短的CTX(Cal)（缺乏ctxAB）前噬菌体的组织是独特的。
