[利用重组自交系群体定位大豆抗虫QTL]

[Mapping insect resistance QTLs of soybean with RIL population].

作者信息

Fu San-Xiong, Wang Hui, Wu Juan-Juan, Liu Hua, Gai Jun-Yi, Yu De-Yue

机构信息

Soybean Research Institute of Nanjing Agricultural University, National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China.

出版信息

Yi Chuan. 2007 Sep;29(9):1139-43. doi: 10.1360/yc-007-1139.

DOI:10.1360/yc-007-1139

PMID:17855266

Abstract

A recombinant inbred line (RIL) population, NJRIKY, which was derived from the cross Kefeng 1 xNannong 1138-2, was used to constructed the genetic linkage map. Larval weight and pupae weight of cotton worm [Prodenia litura (L.) Fabricius] were examined and used as indicators of resistance. Based on the linkage map constructed with SSR markers of this RIL population, one QTL responsible for larval weight was mapped on linkage group G20-O and the position was 31.91 cM. The QTL's additive effect was 0.0408 and explained 11.74 of the total variation of the larval weight. Two QTLs associated with pupae weight were mapped on linkage group G8-D1b+W and G17-L and the positions were 14.71 cM and 0.01 cM, respectively. The QTLs' additive effects were -0.0139 and 0.0103 ,which explained 11.30 and 6.36 of the total variation of larval weight, respectively.

摘要

一个重组自交系（RIL）群体NJRIKY由科丰1号与南农1138-2杂交获得，用于构建遗传连锁图谱。以棉铃虫[斜纹夜蛾（L.）Fabricius]的幼虫体重和蛹重作为抗性指标进行测定。基于该RIL群体的SSR标记构建的连锁图谱，将一个控制幼虫体重的QTL定位在连锁群G20 - O上，位置为31.91 cM。该QTL的加性效应为0.0408，解释了幼虫体重总变异的11.74%。两个与蛹重相关的QTL分别定位在连锁群G8 - D1b + W和G17 - L上，位置分别为14.71 cM和0.01 cM。这两个QTL的加性效应分别为 - 0.0139和0.0103，分别解释了幼虫体重总变异的11.30%和6.36%。