Soybean Research Institute/National Center for Soybean Improvement/MOA Key Laboratory of Biology and Genetic Improvement of Soybean/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
Theor Appl Genet. 2012 Sep;125(5):859-75. doi: 10.1007/s00122-012-1878-7. Epub 2012 May 12.
Bean pyralid (BP; Lamprosema indicata Fabricius) is one of the major leaf-feeding insects that affect soybean crops in central and southern China. Four recombinant inbred line populations (KY, WT, XG and SX) were tested during 2004-2006 in Nanjing, China, to identify quantitative trait loci (QTL) for resistance to BP on the basis of data for rolled leaflet percentage under field infestation conditions. The mapping was performed using QTL Network V2.0 and checked with Windows QTL Cartographer V2.5 and IciMapping V2.2. The results showed that 81-92 % of the phenotypic variation was accounted for by additive QTL (27-43 %), epistatic QTL pairs (5-13 %), and collective unmapped minor QTL (38-58 %). In total, 17 QTL were detected on 11 linkage groups, of which two had additive effects, six had both additive and epistatic effects, and nine had only epistatic effects. Eight epistatic QTL pairs were observed, of which three pairs involved two QTL with additive effects, one involved one QTL with additive effect, and four involved no QTL with additive effects. Different genetic structures for BP resistance were found among the populations. Eight QTL (five additive and three epistatic pairs) were detected in KY, ten QTL (four additive and five epistatic pairs) were detected in WT, and only one additive QTL was detected in both the XG and the SX populations. BP12-1 and BP1-1 are major QTL, with the former accounting for 15, 31, and 50 % of the total genetic variation (including epistasis) in KY, WT, and XG, respectively, and the latter accounting for 13 and 32 % of the total genetic variation in KY and SX, respectively. The additive × year and epistasis × year interaction effects were negligible, indicating that the QTL were stable over the years. Because 41-68 % of the total genetic variation could not be accounted for by these QTL, the use of both identified QTL and unmapped minor QTL in breeding for BP resistance should be considered.
豆荚野螟（BP；Lamprosema indicata Fabricius）是中国中南部大豆作物的主要食叶昆虫之一。在 2004 年至 2006 年期间，在中国南京，使用四个重组自交系群体（KY、WT、XG 和 SX）来测试基于田间侵染条件下卷叶百分率的数据，以鉴定对 BP 的抗性的数量性状位点（QTL）。使用 QTL Network V2.0 进行作图，并使用 Windows QTL Cartographer V2.5 和 IciMapping V2.2 进行检查。结果表明，81-92%的表型变异由加性 QTL（27-43%）、上位性 QTL 对（5-13%）和未定位的小 QTL（38-58%）组成。总共在 11 个连锁群上检测到 17 个 QTL，其中两个具有加性效应，六个具有加性和上位性效应，九个只有上位性效应。观察到 8 个上位性 QTL 对，其中 3 个对涉及两个具有加性效应的 QTL，1 个对涉及一个具有加性效应的 QTL，4 个对涉及没有具有加性效应的 QTL。在不同的群体中发现了对 BP 抗性的不同遗传结构。在 KY 中检测到 8 个 QTL（5 个加性和 3 个上位性对），在 WT 中检测到 10 个 QTL（4 个加性和 5 个上位性对），在 XG 和 SX 群体中仅检测到 1 个加性 QTL。BP12-1 和 BP1-1 是主要的 QTL，前者分别占 KY、WT 和 XG 中总遗传变异（包括上位性）的 15%、31%和 50%，后者分别占 KY 和 SX 中总遗传变异的 13%和 32%。加性×年份和上位性×年份的互作效应可以忽略不计，这表明 QTL 多年来是稳定的。由于这些 QTL 无法解释总遗传变异的 41-68%，因此在对 BP 抗性进行育种时，应考虑同时使用鉴定的 QTL 和未定位的小 QTL。
