Li Wei, Chen Huai-Gu, Li Wei, Zhang Ai-Xiang, Chen Li-Hua, Jiang Wei-Li
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Yi Chuan. 2007 Sep;29(9):1154-60. doi: 10.1360/yc-007-1154.
Simple sequence repeats or microsatellites have been used as genetic markers in population genetics because of their abundance and length variation between different individuals. This study examined the SSRs in the completely sequenced Sclerotinia sclerotiorum and Botrytis cinerea genomes. The occurrences, relative abundance, relative density, most common motifs, and the longest SSRs in the two species were analyzed, and compared with other plant pathogenic fungal species, such as Fusarium graminearum, Magnaporthe grisea and Ustilago maydis. The results demonstrated that the SSRs are abundant in S. sclerotiorum and B. cinerea genomes, and 6 539 and 8 627 SSRs were obtained from these species. The types and distributions of SSRs have similarities between the two species. In the genomes of S. sclerotiorum and B. cinerea, tetra-, penta- and hexa-nucleotide repeats were more abundant than other species, indicating high mutation rates in these species. Furthermore, the abundance and relative density of SSRs were not influenced by the genome sizes and GC content. The analysis in this study provided useful information on applications of microsatellites in population genetics of S. sclerotiorum and B. cinerea.
简单序列重复或微卫星由于其在不同个体间的丰富性和长度变异性，已被用作群体遗传学中的遗传标记。本研究检测了完全测序的核盘菌和灰葡萄孢基因组中的微卫星。分析了这两个物种中微卫星的出现情况、相对丰度、相对密度、最常见基序以及最长的微卫星，并与其他植物病原真菌物种（如禾谷镰刀菌、稻瘟病菌和玉米黑粉菌）进行了比较。结果表明，微卫星在核盘菌和灰葡萄孢基因组中丰富，从这些物种中分别获得了6539个和8627个微卫星。这两个物种中微卫星的类型和分布具有相似性。在核盘菌和灰葡萄孢的基因组中，四核苷酸、五核苷酸和六核苷酸重复比其他物种更为丰富，表明这些物种具有较高的突变率。此外，微卫星的丰度和相对密度不受基因组大小和GC含量的影响。本研究中的分析为微卫星在核盘菌和灰葡萄孢群体遗传学中的应用提供了有用信息。
