通过比较基因组学预测在真菌病原体 Botrytis cinerea 和 Sclerotinia sclerotiorum 中与适应羽扇豆宿主相关的致病性基因。
Prediction of pathogenicity genes involved in adaptation to a lupin host in the fungal pathogens Botrytis cinerea and Sclerotinia sclerotiorum via comparative genomics.
机构信息
UWA School of Agriculture and Environment, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia.
Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, 6102, Australia.
出版信息
BMC Genomics. 2019 May 17;20(1):385. doi: 10.1186/s12864-019-5774-2.
BACKGROUND
Narrow-leafed lupin is an emerging crop of significance in agriculture, livestock feed and human health food. However, its susceptibility to various diseases is a major obstacle towards increased adoption. Sclerotinia sclerotiorum and Botrytis cinerea - both necrotrophs with broad host-ranges - are reported among the top 10 lupin pathogens. Whole-genome sequencing and comparative genomics are useful tools to discover genes responsible for interactions between pathogens and their hosts.
RESULTS
Genomes were assembled for one isolate of B. cinerea and two isolates of S. sclerotiorum, which were isolated from either narrow-leafed or pearl lupin species. Comparative genomics analysis between lupin-derived isolates and others isolated from alternate hosts was used to predict between 94 to 98 effector gene candidates from among their respective non-conserved gene contents.
CONCLUSIONS
Detection of minor differences between relatively recently-diverged isolates, originating from distinct regions and with hosts, may highlight novel or recent gene mutations and losses resulting from host adaptation in broad host-range fungal pathogens.
背景
窄叶羽扇豆是农业、牲畜饲料和人类保健食品中一种具有重要意义的新兴作物。然而，其对各种疾病的易感性是增加采用率的主要障碍。核盘菌和灰葡萄孢菌——两者均为具有广泛宿主范围的坏死营养生物——被列为羽扇豆前 10 大病原体之一。全基因组测序和比较基因组学是发现与病原体与其宿主相互作用相关基因的有用工具。
结果
为来自窄叶羽扇豆或珍珠羽扇豆物种的一种灰葡萄孢菌和两种核盘菌的分离株组装了基因组。对来自不同宿主的羽扇豆分离株和其他分离株的比较基因组学分析，用于预测各自非保守基因含量中 94 到 98 个效应基因候选物。
结论
在起源于不同地区和宿主的相对近期分化的分离株之间检测到较小差异，可能突出了广谱宿主范围真菌病原体在宿主适应过程中导致的新的或近期基因突变和丢失。
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