Legrain V, Michel S, Ortonne J P, Reichert U
Hôpital des Enfants, Bordeaux, France.
Arch Dermatol Res. 1991;283(8):512-5. doi: 10.1007/BF00371924.
Cornified envelopes from the stratum corneum of healthy volunteers and from the involved and uninvolved skin of psoriatic patients were electrophoretically purified, and their peptide composition analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) after cyanogen bromide cleavage. The resulting envelope peptide patterns (EPPs) were compared. In normal subjects, mainly quantitative minor differences in the EPPs were observed between different individuals. In the same individual, palms and soles could be distinguished from other body sites by their EPPs. The palm and sole samples presented identical patterns which were different from the patterns found with samples from other body sites. In psoriatic patients, EPPs of uninvolved skin resembled closely those of healthy epidermis, but showed striking differences from those of lesional skin. The EPPs of psoriatic lesional skin showed a characteristic accumulation of small peptides with molecular weights of 3-11 kDa. The EPP of lesional skin returned to normal during PUVA therapy, indicating that the changes in the biochemical composition of the cornified envelope are correlated with the clinical status of the disease.
对来自健康志愿者角质层以及银屑病患者患部皮肤和非患部皮肤的角化包膜进行电泳纯化，并在溴化氰裂解后通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）分析其肽组成。比较所得的包膜肽图谱（EPPs）。在正常受试者中，不同个体之间的EPPs主要存在数量上的微小差异。在同一个体中，手掌和脚底的EPPs可与身体其他部位区分开来。手掌和脚底样本呈现出相同的图谱，与其他身体部位样本的图谱不同。在银屑病患者中，非患部皮肤的EPPs与健康表皮的EPPs非常相似，但与患部皮肤的EPPs有显著差异。银屑病患部皮肤的EPPs显示出分子量为3-11 kDa的小肽特征性积累。患部皮肤的EPPs在PUVA治疗期间恢复正常，表明角化包膜生化组成的变化与疾病的临床状态相关。
