Birkin J A, Fry J R, Millard L G
Department of Physiology, University Hospital, Queen's Medical Centre, Nottingham, UK.
Clin Exp Dermatol. 1991 Mar;16(2):98-105. doi: 10.1111/j.1365-2230.1991.tb00316.x.
Protein and N-linked glycoprotein biosynthesis was studied in the uninvolved epidermis of patients with psoriasis by the incorporation of radiolabelled leucine and mannose prior to and during PUVA treatment. Analysis of the polyacrylamide gel electrophoresis (PAGE) patterns of the 3[H]-labelled proteins and glycoproteins showed that the major changes in untreated uninvolved psoriatic epidermis compared to normal epidermis were: (a) a shift towards the synthesis of low-molecular-weight glycoproteins; (b) the absence of a 48-kDa peak labelled with mannose; (c) the appearance of 3[H]-mannose-labelled peaks at 40-36 kDa. PUVA treatment gradually changed the PAGE profile back more towards that expected for normal epidermis, with the reintroduction of a 52-48-kDa glycoprotein and reduction of the peaks in the 40-34-kDa region. This effect was dependent on uninterrupted treatment. The PUVA-treated PAGE profiles were compared to those expected in skin tumours (i.e. increased 3[H]-mannose-labelled peaks at 95 and 40-34 kDa with an absence of 62-kDa peaks). It appeared that these criteria were not seen generally as a result of PUVA treatment. However, the results indicate that tumour development may be possible if a patient responds to PUVA treatment by showing an increased peak at 95 and 40-34 kDa in association with a loss of an 3[H]-mannose-labelled peak at 62 kDa.
通过在补骨脂素紫外线A（PUVA）治疗前及治疗期间掺入放射性标记的亮氨酸和甘露糖，对银屑病患者未受累表皮中的蛋白质和N-连接糖蛋白生物合成进行了研究。对3[H]标记的蛋白质和糖蛋白的聚丙烯酰胺凝胶电泳（PAGE）图谱分析表明，与正常表皮相比，未经治疗的未受累银屑病表皮的主要变化有：（a）向低分子量糖蛋白合成方向转变；（b）不存在48 kDa的甘露糖标记峰；（c）在40 - 36 kDa处出现3[H]甘露糖标记峰。PUVA治疗逐渐使PAGE图谱向正常表皮预期的图谱转变，重新出现52 - 48 kDa的糖蛋白，并减少了40 - 34 kDa区域的峰。这种效应依赖于不间断的治疗。将PUVA治疗后的PAGE图谱与皮肤肿瘤预期的图谱进行比较（即95 kDa以及40 - 34 kDa处3[H]甘露糖标记峰增加，且不存在62 kDa峰）。似乎一般不会因PUVA治疗而出现这些标准情况。然而，结果表明，如果患者对PUVA治疗有反应，表现为95 kDa以及40 - 34 kDa处峰增加，同时62 kDa处3[H]甘露糖标记峰缺失，那么肿瘤发生可能是有可能的。
