Schmidt R, Reichert U, Michel S, Shroot B, Bouclier M
FEBS Lett. 1985 Jul 8;186(2):201-4. doi: 10.1016/0014-5793(85)80708-0.
When confluent cultures of the transformed human keratinocyte line SV-K14 are shifted to serum-free medium the cells achieve, within 4 days, the ability to synthesize a cornified envelope after challenge with the Ca2+ ionophore A23187. During these 4 days the enzyme transglutaminase (EC 2.3.2.13), which catalyses the cross-linking of different envelope precursor proteins, is partially transferred from the cytosolic pool into the plasma membrane. The association of the enzyme with the plasma membrane proves to be an essential step in the envelope formation since a direct correlation between plasma membrane-bound transglutaminase and envelope competence is observed. Retinoids block the insertion of the enzyme and therefore prevent envelope formation.
当将转化的人角质形成细胞系SV-K14的汇合培养物转移至无血清培养基时，在用Ca2+离子载体A23187刺激后，细胞在4天内获得合成角质化包膜的能力。在这4天期间，催化不同包膜前体蛋白交联的转谷氨酰胺酶（EC 2.3.2.13）部分地从胞质池转移到质膜中。该酶与质膜的结合被证明是包膜形成中的一个关键步骤，因为观察到质膜结合的转谷氨酰胺酶与包膜形成能力之间存在直接相关性。类视黄醇会阻止该酶的插入，因此会阻止包膜形成。
