Dingfelder R S, Ley D H, McLaren J M, Brownie C
Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Avian Dis. 1991 Oct-Dec;35(4):910-9.
Three-week-old turkeys were inoculated intranasally with approximately 10(6) colony-forming units (CFU) of putative variant Mycoplasma gallisepticum (MG) strains M876, M35, or the virulent S6 reference strain. Uninoculated turkeys in each group served as contact sentinels. The hemagglutination-inhibition (HI) test and enzyme-linked immunosorbent assay (ELISA) were used to determine serologic responses. MG was isolated from 100% and 92% of S6- and M876-inoculated turkeys, respectively, on day 7 PI. However, culture-positive rates among M876-inoculated turkeys declined more rapidly, transmission to contact sentinels took longer and occurred at lower rates, and serologic responses measured by HI and ELISA were lower than in S6-infected turkeys. Testing sera from inoculated turkeys for antibodies to MG in homologous and heterologous ELISA systems indicated that strain M876 was significantly (P less than 0.05) less immunogenic than S6 (days 62 and 95 PI), and that the homologous ELISA was more sensitive (P less than 0.005). MG strain M35 failed to infect turkeys in three attempts, even though the inocula used were viable on culture media.
给3周龄的火鸡经鼻内接种约10(6)个集落形成单位（CFU）的疑似变异鸡败血支原体（MG）菌株M876、M35或强毒株S6参考菌株。每组未接种的火鸡作为接触哨兵。采用血凝抑制（HI）试验和酶联免疫吸附测定（ELISA）来确定血清学反应。在感染后第7天，分别从100%接种S6和92%接种M876的火鸡中分离出MG。然而，接种M876的火鸡中培养阳性率下降更快，向接触哨兵的传播所需时间更长且发生率更低，并且通过HI和ELISA检测的血清学反应低于感染S6的火鸡。在同源和异源ELISA系统中检测接种火鸡血清中针对MG的抗体，结果表明菌株M876的免疫原性明显低于S6（感染后第62天和95天，P<0.05），并且同源ELISA更敏感（P<0.005）。尽管所用接种物在培养基上可存活，但MG菌株M35经三次尝试均未能感染火鸡。
