Blackall P J, Zheng Y Z, Yamaguchi T, Iritani Y, Rogers D G
Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly, Australia.
Avian Dis. 1991 Oct-Dec;35(4):955-9.
A panel of four monoclonal antibodies (MAbs) was evaluated, using a hemagglutination-inhibition test, for its ability to subtype 76 isolates of Haemophilus paragallinarum. The results of the MAb reactions were compared with the results of both the Page and Kume serotyping schemes (the serovars of the Page scheme correspond to the serogroups of the Kume scheme). One MAb (E5C12D10) was raised against a Page serovar A strain and the remaining MAbs (F2E6, D6D8D5, and B3E6F9) against a Page serovar C strain. Six different reaction patterns were found among the 76 isolates of H. paragallinarum. There was total correlation between the MAb reaction pattern and the Page scheme, and thus the Kume scheme, to the serogroup level. All 19 Page serovar A (= Kume serogroup A) strains reacted only with MAb E5C12D10, whereas all five Page serovar B (= Kume serogroup B) strains failed to react with any of the MAbs. All 52 remaining strains were Page serovar C (= Kume serogroup C), and all failed to react with MAb E5C12D10 but showed varying reaction patterns with the three other MAbs. Although the MAbs recognized four subdivisions within Kume serogroup C, these subdivisions differed from the four Kume C serovars. This panel of MAbs can be used to assign isolates of H. paragallinarum to either Page serovars or Kume serogroups. Although the subdivisions recognized by the MAbs within the Page serovar C strains do not correspond to the Kume serovars, they may be useful in epidemiological applications.
使用血凝抑制试验评估了一组四种单克隆抗体（MAb）对76株副鸡嗜血杆菌进行亚型分型的能力。将MAb反应结果与Page和久米血清分型方案的结果进行了比较（Page方案的血清型对应于久米方案的血清群）。一种MAb（E5C12D10）是针对Page血清型A菌株产生的，其余的MAb（F2E6、D6D8D5和B3E6F9）是针对Page血清型C菌株产生的。在76株副鸡嗜血杆菌中发现了六种不同的反应模式。MAb反应模式与Page方案以及久米方案在血清群水平上完全相关。所有19株Page血清型A（=久米血清群A）菌株仅与MAb E5C12D10反应，而所有5株Page血清型B（=久米血清群B）菌株均未与任何一种MAb反应。其余所有52株菌株均为Page血清型C（=久米血清群C），且均未与MAb E5C12D10反应，但与其他三种MAb表现出不同的反应模式。尽管MAb识别出久米血清群C内的四个亚群，但这些亚群与久米C血清型不同。这组MAb可用于将副鸡嗜血杆菌分离株归类为Page血清型或久米血清群。尽管MAb在Page血清型C菌株中识别出的亚群与久米血清型不对应，但它们可能在流行病学应用中有用。
