Blackall P J, Zheng Y Z, Takagi M, Terzolo H R, Sandoval V E, Silva E N
Queensland Department of Primary Industries, Animal Research Institute, Australia.
Avian Dis. 1994 Apr-Jun;38(2):361-5.
Two monoclonal antibodies (MAbs) raised against a serovar A Haemophilus paragallinarum were evaluated for their ability to react with 11 reference strains that represented all the recognized serovars and with 27 field isolates of Page serovar A collected from around the world. The MAbs were used in a hemagglutination-inhibition assay. Both MAbs recognized type strains of Page serovar A and Kume serovars A-1 and A-2 but not the type strains of Kume serovars A-3 and A-4. Neither MAb recognized the type strains of Page serovars B and C or Kume serovars B-1, C-1, C-2, C-3, or C-4. When evaluated with the 27 Page serovar A field isolates, both MAbs recognized only 10 isolates. All of the recognized isolates belonged to Kume serovars A-1 (nine isolates) or A-2 (one isolate). All of the field isolates that were not recognized by one or the other of the MAbs either were Kume serovar A-4 (seven isolates) or could not be placed in an existing Kume A serovar (10 isolates). The results indicate that the epitope recognized by these MAbs is present only in strains of H. paragallinarum that belong to Kume serovars A-1 and A-2.
对两种针对副鸡嗜血杆菌A血清型产生的单克隆抗体（MAb）进行了评估，以检测它们与代表所有已识别血清型的11株参考菌株以及从世界各地收集的27株Page A血清型的现场分离株发生反应的能力。这些单克隆抗体用于血凝抑制试验。两种单克隆抗体均识别Page A血清型以及久米A-1和A-2血清型的模式菌株，但不识别久米A-3和A-4血清型的模式菌株。两种单克隆抗体均未识别Page B和C血清型或久米B-1、C-1、C-2、C-3或C-4血清型的模式菌株。当用27株Page A血清型的现场分离株进行评估时，两种单克隆抗体仅识别10株分离株。所有被识别的分离株均属于久米A-1血清型（9株）或A-2血清型（1株）。未被任何一种单克隆抗体识别的所有现场分离株要么是久米A-4血清型（7株），要么无法归入现有的久米A血清型（10株）。结果表明，这些单克隆抗体识别的表位仅存在于属于久米A-1和A-2血清型的副鸡嗜血杆菌菌株中。
