Yagi T, Goto M, Nakano K, Kimura K, Inokuchi H
J Biochem. 1975 Sep;78(3):443-54. doi: 10.1093/oxfordjournals.jbchem.a130927.
A new assay method for hydrogenase [EC 1.12.2.1] based on the enzymic electrode reaction of H2-H+ equilibrium has been established. The method is based on the experimental fact that the short-circuit current of the electric cell composed of an electrode with hydrogenase and methylviologen as the mediator of H2-H+ equilibrium and a saturated calomel electrode as the counter electrode, is practically proportional to the amount of hydrogenase in the cell. The new method is referred to as the "enzymic electric cell method." This technique has applications not only to routine activity assay but also to the direct determination of the time course of enzyme denaturation, which has not previously been possible.
基于H₂-H⁺平衡的酶电极反应，建立了一种新的氢化酶[EC 1.12.2.1]测定方法。该方法基于这样一个实验事实：由含有氢化酶和作为H₂-H⁺平衡介质的甲基紫精的电极与作为对电极的饱和甘汞电极组成的电池的短路电流，实际上与电池中氢化酶的量成正比。这种新方法被称为“酶电池法”。该技术不仅可应用于常规活性测定，还可直接测定酶变性的时间进程，而这在以前是不可能的。
