Brandt B L, Kimes B W, Klier F G
J Cell Physiol. 1976 Jul;88(3):255-75. doi: 10.1002/jcp.1040880302.
The morphological, ultrastructural, biochemical and electrophysiological properties of B104-F, a clonal cell line derived from a nitrosoethylurea-induced neoplasm in a rat, were studied as a function of the growth phase of the culture. Cells in exponentially growing cultures are mononucleate and produce action potentials when stimulated electrically. Stationary phase cultures contain three types of cells: cells of the first type are mononucleate and have long processes containing microfilaments and many parallel microtubules; cells of the second type are mononucleate but contain no microtubules and few microfilaments; and cells of the third type have ultrastructural features typical of multinucleate, striated myotubes. Multinucleate cells generate action potentials with both sodium and calcium components and are depolarized by acetylcholine. The acetylcholine response is blocked by d-tubocurarine. The specific activity of creatine phosphokinase is nine times higher in stationary phase cultures than in exponentially growing ones while the myokinase specific activity is unchanged. The gamma-aminobutyric acid content of the cells is 3.5- to 26-fold higher in stationary phase than in exponentially growing cultures, depending on the degree of fusion of the culture. The properties of B104-F are discussed in relation to the properties of developing skeletal muscle and of central nervous system cell lines.
对B104-F（一种源自大鼠亚硝基乙基脲诱导肿瘤的克隆细胞系）的形态学、超微结构、生化和电生理特性进行了研究，研究内容为其作为培养生长阶段的函数。指数生长期培养物中的细胞为单核细胞，电刺激时可产生动作电位。稳定期培养物包含三种类型的细胞：第一类细胞为单核细胞，具有含有微丝和许多平行微管的长突起；第二类细胞为单核细胞，但不含微管且微丝很少；第三类细胞具有多核横纹肌管的典型超微结构特征。多核细胞产生具有钠和钙成分的动作电位，并被乙酰胆碱去极化。乙酰胆碱反应可被d -筒箭毒碱阻断。磷酸肌酸激酶的比活性在稳定期培养物中比在指数生长期培养物中高九倍，而肌激酶比活性不变。细胞内γ-氨基丁酸含量在稳定期比指数生长期培养物高3.5至26倍，具体倍数取决于培养物的融合程度。结合发育中的骨骼肌和中枢神经系统细胞系的特性，对B104-F的特性进行了讨论。
