体外克隆性成肌细胞上的乙酰胆碱反应。
Acetylcholine responses on clonal myogenic cells in vitro.
作者信息
Steinbach J H
出版信息
J Physiol. 1975 May;247(2):393-405. doi: 10.1113/jphysiol.1975.sp010937.
Abstract
- Some electrophysiological properties of the acetylcholine response shown by clonal rat myogenic cells were studied in vitro. Some mononucleate myoblasts showed a slow hyperpolarizing response. 2. Multinucleate myotubes showed a fast depolarizing response that was very similar to that of mammalian skeletal muscle in vivo, and differed from the hyperpolarizing response of myoblasts. 3. The depolarizing response involved Na, K and Ca ions, but not Cl ions. The dependence of the reversal potential on external ion concentrations was generally similar to that shown by frog muscle, but some quantitative differences were seen. 4. The reversal potential determined at sites of nerve-muscle interaction did not differ from those at background or non-interacted sites.
摘要
- 对克隆大鼠肌源性细胞所表现出的乙酰胆碱反应的一些电生理特性进行了体外研究。一些单核成肌细胞表现出缓慢的超极化反应。2. 多核肌管表现出快速的去极化反应，这与体内哺乳动物骨骼肌的反应非常相似，且与成肌细胞的超极化反应不同。3. 去极化反应涉及钠、钾和钙离子，但不涉及氯离子。反转电位对外部离子浓度的依赖性通常与蛙肌所表现出的相似，但也存在一些定量差异。4. 在神经 - 肌肉相互作用部位测定的反转电位与背景或未相互作用部位的反转电位没有差异。
相似文献
1
Acetylcholine responses on clonal myogenic cells in vitro.体外克隆性成肌细胞上的乙酰胆碱反应。
J Physiol. 1975 May;247(2):393-405. doi: 10.1113/jphysiol.1975.sp010937.
2
Sodium and calcium components of the action potential in a developing skeletal muscle cell line.发育中的骨骼肌细胞系动作电位的钠和钙成分
J Physiol. 1975 Jan;244(1):145-59. doi: 10.1113/jphysiol.1975.sp010788.
3
[Ionic mechanisms of the rapid (nicotinic) phase of the acetylcholine response of identified Planorbis neurons].
Neirofiziologiia. 1980;12(5):533-40.
4
Physiological and kinetic properties of cholinergic receptors activated by multiaction interneurons in buccal ganglia of Aplysia.海兔口腔神经节中多作用中间神经元激活的胆碱能受体的生理和动力学特性。
J Neurophysiol. 1977 Mar;40(2):333-48. doi: 10.1152/jn.1977.40.2.333.
5
Developmental changes of membrane electrical properties in a rat skeletal muscle cell line.大鼠骨骼肌细胞系中膜电特性的发育变化
J Physiol. 1975 Jan;244(1):129-43. doi: 10.1113/jphysiol.1975.sp010787.
6
Development of a clonal myogenic cell line with unusual biochemical properties.具有异常生化特性的克隆性成肌细胞系的建立。
J Cell Physiol. 1976 Jul;88(3):255-75. doi: 10.1002/jcp.1040880302.
7
Electrophysiological properties of the membrane and acetylcholine receptor in developing rat and chick myotubes.发育中大鼠和鸡肌管的膜及乙酰胆碱受体的电生理特性
J Gen Physiol. 1975 Sep;66(3):327-55. doi: 10.1085/jgp.66.3.327.
8
Ionic properties of the acetylcholine receptor in cultured rat myotubes.培养的大鼠肌管中乙酰胆碱受体的离子特性
J Gen Physiol. 1975 Jun;65(6):751-67. doi: 10.1085/jgp.65.6.751.
9
Pancreatic acinar cells: the acetylcholine equilibrium potential and its ionic dependency.胰腺腺泡细胞：乙酰胆碱平衡电位及其离子依赖性。
J Physiol. 1977 Aug;269(3):735-51. doi: 10.1113/jphysiol.1977.sp011926.
10
Pancreatic acinar cells: ionic dependence of acetylcholine-induced membrane potential and resistance change.胰腺腺泡细胞：乙酰胆碱诱导的膜电位和电阻变化的离子依赖性
J Physiol. 1975 Jan;244(2):431-65. doi: 10.1113/jphysiol.1975.sp010807.
引用本文的文献
1
Supramolecular therapeutics to treat the side effects induced by a depolarizing neuromuscular blocking agent.超分子疗法治疗去极化型神经肌肉阻滞剂引起的副作用。
Theranostics. 2019 May 18;9(11):3107-3121. doi: 10.7150/thno.34947. eCollection 2019.
2
Voltage clamp analysis of the effect of cationic substitution on the conductance of end-plate channels.阳离子替代对终板通道电导影响的电压钳分析
Pflugers Arch. 1982 Jul;394(1):38-47. doi: 10.1007/BF01108306.
3
Pharmacologic characterization of the Na+ ionophores in L6 myotubes.L6肌管中Na⁺离子载体的药理学特性
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1355-9. doi: 10.1073/pnas.73.4.1355.
4
Acetylcholine receptor distribution on myotubes in culture correlated to acetylcholine sensitivity.培养的肌管上乙酰胆碱受体的分布与乙酰胆碱敏感性相关。
J Physiol. 1977 Jul;269(1):155-76. doi: 10.1113/jphysiol.1977.sp011897.
5
The distribution of acetylcholine receptors on muscle fibres of regenerating salamander limbs.乙酰胆碱受体在蝾螈再生肢体肌肉纤维上的分布。
J Physiol. 1977 Apr;266(3):765-76. doi: 10.1113/jphysiol.1977.sp011792.
6
Ionic basis of transient inward current induced by strophanthidin in cardiac Purkinje fibres.毒毛花苷诱导心脏浦肯野纤维瞬时内向电流的离子基础。
J Physiol. 1978 Aug;281:209-26. doi: 10.1113/jphysiol.1978.sp012417.
7
Ion-concentration dependence of the reversal potential and the single channel conductance of ion channels at the frog neuromuscular junction.蛙神经肌肉接头处离子通道的反转电位和单通道电导的离子浓度依赖性。
J Physiol. 1979 Jan;286:417-45. doi: 10.1113/jphysiol.1979.sp012629.
本文引用的文献
1
The acetylcholine sensitivity of frog muscle fibres after complete or partial devervation.完全或部分去神经支配后青蛙肌肉纤维的乙酰胆碱敏感性
J Physiol. 1960 Apr;151(1):1-23.
2
Steps in the neoplastic transformation of hamster embryo cells by polyoma virus.多瘤病毒使仓鼠胚胎细胞发生肿瘤转化的步骤。
Proc Natl Acad Sci U S A. 1963 Feb 15;49(2):171-9. doi: 10.1073/pnas.49.2.171.
3
Effects of calcium on the conductance change of the end-plate membrane during the action of transmitter.钙对递质作用期间终板膜电导变化的影响。
J Physiol. 1963 Jun;167(1):141-55. doi: 10.1113/jphysiol.1963.sp007137.
4
Some properties of conductance changes at the end-plate membrane during the action of acetylcholine.乙酰胆碱作用期间终板膜电导变化的一些特性。
J Physiol. 1963 Jun;167(1):128-40. doi: 10.1113/jphysiol.1963.sp007136.
5
On the permeability of end-plate membrane during the action of transmitter.递质作用期间终板膜的通透性
J Physiol. 1960 Nov;154(1):52-67. doi: 10.1113/jphysiol.1960.sp006564.
6
A study of supersensitivity in denervated mammalian skeletal muscle.失神经支配的哺乳动物骨骼肌超敏反应的研究。
J Physiol. 1959 Jun 23;147(1):178-93. doi: 10.1113/jphysiol.1959.sp006233.
7
Effects of methyl- and ethyl-derivatives of NH4 on the neuromuscular junction.NH4的甲基和乙基衍生物对神经肌肉接头的影响。
Jpn J Physiol. 1959 Jun 25;9(2):130-42. doi: 10.2170/jjphysiol.9.130.
8
On the factors which determine the amplitude of the miniature end-plate potential.论决定微小终板电位幅度的因素。
J Physiol. 1957 Jul 11;137(2):267-78. doi: 10.1113/jphysiol.1957.sp005811.
9
Influence of phospholipase C on some electrical properties of the skeletal muscle membrane.磷脂酶C对骨骼肌膜某些电特性的影响。
J Physiol. 1967 May;190(1):123-37. doi: 10.1113/jphysiol.1967.sp008197.
10
Muscle postjunctional membrane: changes in chemosensitivity produced by calcium.肌肉终板膜：钙引起的化学敏感性变化。
Science. 1966 Oct 14;154(3746):266-7. doi: 10.1126/science.154.3746.266.
Zotero 插件