Yasmeen Talat, Qureshi Ghulam Sarwar, Perveen Sughra
Department of Anatomy, Sindh Medical College, Jinnah Postgraduate Medical Centre, Karachi.
J Pak Med Assoc. 2007 Jul;57(7):349-51.
To see the toxic effects of NSAID on kidney tissue of albino rats.
For this experimental study, 16 albino rats were taken. They were divided into two groups; A and B. The animals in group-A were given Normal Saline. Group-B received Diclofenac Sodium 2 mg/kg/day by feeding tube for 14 days. On day-15 all animals were sacrificed. Kidneys were removed, fixed, embedded in paraffin, section cut at 4 microm thick and stained with H&E, PAS, and silver methenamine. Renal histology was done under light microscope to see the renal tubular diameter, count and cellular detail.
The result of present study revealed that diclofenac sodium in single daily dose of 2 mg/kg for a period of two weeks effectively produced destruction of proximal and distal convoluted tubules in adult albino rats showing the dilatation of tubule and flattening of tubular epithelium, disruption of brush border in proximal tubule and thickening of basement membrane around proximal and distal tubular epithelium.
Diclofenac sodium induced nephrotoxicity causes the destruction of proximal and distal convoluted tubules showing the dilatation of tubule and flattening of its epithelium in albino rats.
观察非甾体抗炎药（NSAID）对白化病大鼠肾脏组织的毒性作用。
在本实验研究中，选取16只白化病大鼠。将它们分为两组：A组和B组。A组动物给予生理盐水。B组通过喂食管给予双氯芬酸钠2毫克/千克/天，持续14天。在第15天，处死所有动物。取出肾脏，固定，石蜡包埋，切成4微米厚的切片，并用苏木精-伊红（H&E）、过碘酸雪夫（PAS）和亚甲胺银染色。在光学显微镜下进行肾脏组织学检查，以观察肾小管直径、计数和细胞细节。
本研究结果显示，连续两周每日单剂量给予2毫克/千克双氯芬酸钠可有效导致成年白化病大鼠近端和远端曲管的破坏，表现为肾小管扩张、肾小管上皮扁平、近端小管刷状缘破坏以及近端和远端肾小管上皮周围基底膜增厚。
双氯芬酸钠诱导的肾毒性导致白化病大鼠近端和远端曲管的破坏，表现为肾小管扩张及其上皮扁平。
