Mousa Ahmed Abdelmoniem, Elweza Ahmed Essam, Elbaz Hamed Talaat, Tahoun Enas Abd El-Aziz, Shoghy Khaled Mohamed, Elsayed Islam, Hassan El Barbary
Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, Menoufia, Egypt.
Department of Theriogenology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 32897, Menoufia, Egypt.
J Tradit Complement Med. 2019 Nov 25;10(6):521-528. doi: 10.1016/j.jtcme.2019.11.002. eCollection 2020 Nov.
The current study was conducted to investigate the protective properties of leaves methanolic extract (EGLME) against diclofenac sodium (DS) induced hepatorenal and testicular toxicity in male rats. A total of 40 rats were equally divided into 4 groups, Control, Diclofenac sodium (DS), EGLME and DS + EGLME groups, respectively. DS and EGLME were administered orally at dose rate 2.5 and 100 mg/kg BW, 4 times/week for 8 weeks, respectively. Administration of DS distorted hepatorenal functions manifested by alteration of serum levels of ALT, AST, total protein and albumin, creatinine and urea with changes of histological architectures. DS caused reproductive toxicity represented by minimized sperm count, individual sperm motility and viability; depleted concentration of reduced glutathione (GSH) in testicular tissue; and decreased testosterone level with alteration in testicular histological features. In contrast, co-treatment of DS intoxicated rats with EGLME protected rats against the adverse effects of DS revealing enhancing properties of EGLME on rats' liver, kidney and testes. In conclusion, we demonstrated that EGLME had a potent protecting property against DS induced hepatic, renal and testicular toxicity in male rats, with special concern to testicular tissue via modulation of GSH as an oxidant marker.
(classification by EVISE): Diclofenac sodium toxicity (hepatorenal and testicular toxicity), co-treatment with natural herbal extract, blood biochemical assays, tissue anti-oxidants assay, histopathology and reproductive indices analyses.
本研究旨在探讨叶甲醇提取物（EGLME）对双氯芬酸钠（DS）诱导的雄性大鼠肝肾和睾丸毒性的保护作用。总共40只大鼠被平均分为4组，分别为对照组、双氯芬酸钠（DS）组、EGLME组和DS + EGLME组。DS和EGLME分别以2.5和100 mg/kg体重的剂量口服给药，每周4次，共8周。DS的给药导致肝肾功 能紊乱，表现为血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、总蛋白、白蛋白、肌酐和尿素水平的改变以及组织结构的变化。DS引起生殖毒性，表现为精子数量减少、个体精子活力和生存力降低；睾丸组织中还原型谷胱甘肽（GSH）浓度降低；睾酮水平下降以及睾丸组织学特征改变。相比之下，用EGLME对DS中毒大鼠进行联合治疗可保护大鼠免受DS的不良影响，显示出EGLME对大鼠肝脏、肾脏和睾丸的增强作用。总之，我们证明EGLME对DS诱导的雄性大鼠肝、肾和睾丸毒性具有强大的保护作用，特别关注通过调节作为氧化标志物的GSH对睾丸组织的保护作用。
（由EVISE分类）：双氯芬酸钠毒性（肝肾和睾丸毒性）、与天然草药提取物联合治疗、血液生化测定、组织抗氧化剂测定、组织病理学和生殖指标分析。
