Kovács Beáta Marianna, Toussaint Mathilda J M, Gruys E, Fábián Ibolya B, Szilágyi L, Janan J, Rudas P
Department of Physiology and Biochemistry, Faculty of Veterinary Science, Szent Istvin University, H-1078 Budapest, István u. 2, Hungary.
Acta Vet Hung. 2007 Sep;55(3):349-57. doi: 10.1556/AVet.55.2007.3.9.
Serum amyloid A (SAA) is of interest as the circulating precursor of amyloid A protein, the fibrillar component of AA (secondary) amyloid deposits, and also as an extremely sensitive and rapid major acute phase protein. Serum concentrations of acute phase proteins (APPs) provide valuable information about the diagnosis and prognosis of various diseases, and thus the relevance of APPs for monitoring the health status of domestic animals is widely accepted. More importantly, the measurement of SAA concentration assists in assessing the prognosis in secondary amyloidosis, which is a common disease of geese, affecting an increasing number of animals. In the present study we introduce a highly sensitive goose-specific ELISA method for measuring SAA concentration in goose serum or plasma samples. Samples were taken from geese of the Landes Grey and Hungarian White breeds, which were stimulated for an acute phase reaction by administration of a commercially available fowl cholera vaccine containing inactivated Pasteurella multocida. Strong and characteristically rapid acute phase responses were measured in both breeds, peaking at approximately 24 h after inoculation. The maximum SAA concentration was 1200 microg/ml. At 72 h postinoculation, the concentrations returned to pre-inoculation values. There was significantly (p = 0.004) less intense response in the control groups; however, a very mild increase of SAA levels was detected due to the stress inevitably caused by the sampling procedure.
血清淀粉样蛋白A（SAA）作为淀粉样蛋白A的循环前体备受关注，淀粉样蛋白A是AA（继发性）淀粉样沉积物的纤维成分，同时也是一种极其敏感且快速反应的主要急性期蛋白。急性期蛋白（APPs）的血清浓度可为各种疾病的诊断和预后提供有价值的信息，因此APPs对于监测家畜健康状况的相关性已被广泛认可。更重要的是，SAA浓度的测定有助于评估继发性淀粉样变性的预后，继发性淀粉样变性是鹅的一种常见疾病，影响着越来越多的动物。在本研究中，我们介绍了一种高度敏感的鹅特异性ELISA方法，用于测定鹅血清或血浆样本中的SAA浓度。样本取自朗德灰鹅和匈牙利白鹅，通过接种含有灭活多杀性巴氏杆菌的市售禽霍乱疫苗刺激其发生急性期反应。两个品种均检测到强烈且典型的快速急性期反应，在接种后约24小时达到峰值。最大SAA浓度为1200μg/ml。接种后72小时，浓度恢复到接种前水平。对照组的反应强度明显较低（p = 0.004）；然而，由于采样过程不可避免地造成的应激，检测到SAA水平有非常轻微的升高。
