Avula B, Dentali S, Khan I A
National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, 38667 USA.
Pharmazie. 2007 Aug;62(8):593-6.
A HPLC method has been developed which permits the quantification of methyl paraben, benzethonium chloride and triclosan in various samples of grapefruit seed extract (GSE). The best results were obtained with a Phenomenex Gemini C18 column using gradient mobile phase of water (0.1% acetic acid) and acetonitrile (0.1% acetic acid) with a flow rate of 1.0 mL per minute. The detection wavelength was 254 nm for methyl paraben, and 275 nm for benzethonium chloride and triclosan. The main synthetic antimicrobial agent identified in commercial GSE samples was benzethonium chloride in concentrations from 0.29-21.84%. Positive ion electrospray MS of a commercial GSE sample showed a molecular ion at m/z 412 [M+], which matched that of a standard of benzethonium chloride. Triclosan was detected in two samples at 0.009 and 1.13%concentrations; while methyl paraben was not detected in the samples analyzed.
已开发出一种高效液相色谱（HPLC）方法，可对葡萄柚籽提取物（GSE）的各种样品中的对羟基苯甲酸甲酯、苄索氯铵和三氯生进行定量分析。使用菲罗门Gemini C18色谱柱，以水（0.1%乙酸）和乙腈（0.1%乙酸）为梯度流动相，流速为每分钟1.0 mL时，可获得最佳结果。对羟基苯甲酸甲酯的检测波长为254 nm，苄索氯铵和三氯生的检测波长为275 nm。在商业GSE样品中鉴定出的主要合成抗菌剂是苄索氯铵，浓度为0.29 - 21.84%。一个商业GSE样品的正离子电喷雾质谱显示，质荷比为412 [M+]的分子离子，与苄索氯铵标准品的分子离子相匹配。在两个样品中检测到三氯生，浓度分别为0.009%和1.13%；而在所分析的样品中未检测到对羟基苯甲酸甲酯。
