Departamento de Química - CFM, Grupo de Estudos de Processos Eletroquímicos e Eletroanalíticos, Centro de Ciências Físicas e Matemáticas - CFM, Campus Universitário Reitor João David Ferreira Lima, Universidade Federal de Santa Catarina - UFSC, Florianópolis, SC, 88040-900, Brazil.
Mikrochim Acta. 2020 Mar 28;187(4):250. doi: 10.1007/s00604-020-04225-7.
Two analytical methods were developed using electrochemical and spectrometric techniques for the simultaneous determination of endocrine disruptors triclosan and methylparaben in the monitoring of personal care products. For the electroanalytical analyses, a sensitive electrode based on graphene quantum dots supported in chitosan was employed. Under optimized conditions and a working potential of typically + 0.60 V for triclosan and + 0.81 V (vs. Ag/AgCl) for methylparaben, the calibration plots obtained by differential pulse voltammetry were linear in the range 0.10 to 10.0 μmol L. The detection limits were 0.03 and 0.04 μmol L for triclosan and methylparaben, respectively. For the spectrometric method, UV/VIS spectrometry was used with a mathematical processing of non-linear deconvolution. This processing was used to solve the problem of overlapping absorption bands of triclosan (282 nm) and methylparaben (257 nm), which enabled simultaneous determination. The calibration plots by UV/VIS spectrometry were linear in the range 1.0 to 14.0 μmol L with detection limits of 0.42 and 0.37 μmol L, respectively, for triclosan and methylparaben. Similar results obtained from the calibration plots of individual analytes suggest that the methods can be applied for individual or simultaneous determination of these species. Both methods were employed in the analysis of five samples of personal care products: toothpaste, antiseptic soap, antiseptic deodorant, shampoo, and a bath kit (soap and shampoo). The statistical tests indicated that there were no significant differences regarding the accuracy and precision of the data provided by the two methods described herein. Graphical abstract Schematic representation for simultaneous determination of triclosan and methylparaben: electrochemical method employing an electrode modified with graphene quantum dots supported in chitosan and spectrometric method applying a non-linear deconvolution of spectrum.
两种分析方法，即电化学和光谱技术，被开发用于内分泌干扰物三氯生和甲基对羟基苯甲酸酯在个人护理产品监测中的同时测定。对于电分析，使用了基于壳聚糖支撑的石墨烯量子点的灵敏电极。在优化条件下，工作电位通常为三氯生的+0.60 V（相对于 Ag/AgCl）和甲基对羟基苯甲酸酯的+0.81 V（相对于 Ag/AgCl），通过差分脉冲伏安法获得的校准曲线在 0.10 至 10.0 μmol·L 范围内呈线性。三氯生和甲基对羟基苯甲酸酯的检测限分别为 0.03 和 0.04 μmol·L。对于光谱方法，使用了紫外/可见光谱法，并对非线性去卷积进行了数学处理。该处理用于解决三氯生（282 nm）和甲基对羟基苯甲酸酯（257 nm）吸收带重叠的问题，从而实现了同时测定。紫外/可见光谱法的校准曲线在 1.0 至 14.0 μmol·L 范围内呈线性，三氯生和甲基对羟基苯甲酸酯的检测限分别为 0.42 和 0.37 μmol·L。从单个分析物的校准曲线得到的相似结果表明，该方法可用于这些物质的单独或同时测定。两种方法均用于分析五个个人护理产品样品：牙膏、抗菌皂、抗菌除臭剂、洗发水和洗浴套件（肥皂和洗发水）。统计检验表明，两种方法提供的数据在准确性和精密度方面没有显著差异。
