Takeoka Gary R, Dao Lan T, Wong Rosalind Y, Harden Leslie A
Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, California 94710, USA.
J Agric Food Chem. 2005 Sep 21;53(19):7630-6. doi: 10.1021/jf0514064.
Commercial grapefruit seed extracts (GSE) were extracted with chloroform. The solvent was evaporated, and the resulting solid was subsequently analyzed by high-performance liquid chromatography (HPLC), electrospray ionization mass spectrometry (ESI/MS), tandem mass spectrometry (ESI/MS/MS), and elemental analysis (by proton-induced X-ray emission analysis). Three major constituents were observed by HPLC and were identified as benzyldimethyldodecylammonium chloride, benzyldimethyltetradecylammonium chloride, and benzyldimethylhexadecylammonium chloride. This mixture of homologues is commonly known as benzalkonium chloride, a widely used synthetic antimicrobial ingredient used in cleaning and disinfection agents.
商业葡萄柚籽提取物（GSE）用氯仿进行萃取。将溶剂蒸发后，所得固体随后通过高效液相色谱法（HPLC）、电喷雾电离质谱法（ESI/MS）、串联质谱法（ESI/MS/MS）以及元素分析（质子激发X射线发射分析）进行分析。通过HPLC观察到三种主要成分，经鉴定分别为苄基二甲基十二烷基氯化铵、苄基二甲基十四烷基氯化铵和苄基二甲基十六烷基氯化铵。这种同系物混合物通常被称为苯扎氯铵，是一种广泛用于清洁和消毒剂中的合成抗菌成分。
