Warger William C, Laevsky Gary S, Townsend Daniel J, Rajadhyaksha Milind, DiMarzio Charles A
Northeastern University, The Bernard M. Gordon Center for Subsurface Sensing and Imaging Systems, Boston, Massachusetts 02115, USA.
J Biomed Opt. 2007 Jul-Aug;12(4):044006. doi: 10.1117/1.2753744.
We present a multimodal optical microscope that incorporates six imaging modalities on one common platform. The imaging modalities include three staring modes, optical quadrature microscopy (OQM), differential interference contrast (DIC) microscopy, and epi-fluorescence microscopy, and three scanning modes, confocal reflectance microscopy (CRM), confocal fluorescence microscopy (CFM), and two-photon microscopy (2PM). OQM reconstructs the amplitude and phase of an optically transparent specimen within a modified Mach-Zehnder configuration. DIC microscopy images the phase gradient along a specified direction of an optically transparent specimen. CRM detects index of refraction changes that modulate backscatter. Epi-fluorescence microscopy, CFM, and 2PM detect endogenous and exogenous fluorophores within a specimen. The scanning modes are inherently capable of producing three-dimensional (3-D) images due to optical sectioning and localized probing. Illumination and imaging are performed coaxially with minimal changes of optical components between modes. Multimodal images of embryos are shown to demonstrate the microscope's imaging capabilities.
我们展示了一种多模态光学显微镜，它在一个通用平台上集成了六种成像模态。这些成像模态包括三种凝视模式，即光学正交显微镜（OQM）、微分干涉对比（DIC）显微镜和落射荧光显微镜，以及三种扫描模式，即共焦反射显微镜（CRM）、共焦荧光显微镜（CFM）和双光子显微镜（2PM）。OQM在改进的马赫曾德尔配置内重建光学透明样本的振幅和相位。DIC显微镜对光学透明样本沿指定方向的相位梯度进行成像。CRM检测调制后向散射的折射率变化。落射荧光显微镜、CFM和2PM检测样本内的内源性和外源性荧光团。由于光学切片和局部探测，扫描模式本质上能够生成三维（3-D）图像。照明和成像同轴进行，各模式之间光学组件的变化最小。展示了胚胎的多模态图像以证明显微镜的成像能力。
