三维荧光样本的扫描全息显微镜技术
Scanning holographic microscopy of three-dimensional fluorescent specimens.
作者信息
Indebetouw Guy, Zhong Wenwei
机构信息
Department of Physics, Virginia Tech, Blacksburg, Virginia 24061-0435, USA.
出版信息
J Opt Soc Am A Opt Image Sci Vis. 2006 Jul;23(7):1699-707. doi: 10.1364/josaa.23.001699.
Abstract
We demonstrate experimentally the three-dimensional reconstructions of fluorescent biological specimens using scanning holographic microscopy. Three-dimensional reconstructions with transverse resolution below about 1 microm of transmission and fluorescence emission images are presented and analyzed. The limitations of the method are discussed.
摘要
我们通过扫描全息显微镜对荧光生物样本进行了三维重建的实验演示。展示并分析了横向分辨率低于约1微米的透射和荧光发射图像的三维重建。讨论了该方法的局限性。
相似文献
1
Scanning holographic microscopy of three-dimensional fluorescent specimens.
J Opt Soc Am A Opt Image Sci Vis. 2006 Jul;23(7):1699-707. doi: 10.1364/josaa.23.001699.
2
Point-spread function synthesis in scanning holographic microscopy.
J Opt Soc Am A Opt Image Sci Vis. 2006 Jul;23(7):1708-17. doi: 10.1364/josaa.23.001708.
3
Scanning holographic microscopy with transverse resolution exceeding the Rayleigh limit and extended depth of focus.
J Opt Soc Am A Opt Image Sci Vis. 2005 May;22(5):892-8. doi: 10.1364/josaa.22.000892.
4
A posteriori quasi-sectioning of the three-dimensional reconstructions of scanning holographic microscopy.
J Opt Soc Am A Opt Image Sci Vis. 2006 Oct;23(10):2657-61. doi: 10.1364/josaa.23.002657.
5
Digital confocal microscope.
Opt Express. 2012 Sep 24;20(20):22720-7. doi: 10.1364/OE.20.022720.
6
Guiding a confocal microscope by single fluorescent nanoparticles.
Opt Lett. 2007 Sep 15;32(18):2729-31. doi: 10.1364/ol.32.002729.
7
Automatic method for focusing biological specimens in digital lensless holographic microscopy.
Opt Lett. 2014 May 1;39(9):2569-72. doi: 10.1364/OL.39.002569.
8
High-speed confocal fluorescence imaging with a novel line scanning microscope.
J Biomed Opt. 2006 Nov-Dec;11(6):064011. doi: 10.1117/1.2402110.
9
Low-cost, scalable laser scanning module for real-time reflectance and fluorescence confocal microscopy.
Appl Opt. 2005 Apr 10;44(11):2013-8. doi: 10.1364/ao.44.002013.
10
Hyperspectral confocal microscope.
Appl Opt. 2006 Aug 20;45(24):6283-91. doi: 10.1364/ao.45.006283.
引用本文的文献
1
Digital holography and its multidimensional imaging applications: a review.
Microscopy (Oxf). 2018 Apr 1;67(2):55-67. doi: 10.1093/jmicro/dfy007.
2
Signal enhanced holographic fluorescence microscopy with guide-star reconstruction.
Biomed Opt Express. 2016 Mar 14;7(4):1271-83. doi: 10.1364/BOE.7.001271. eCollection 2016 Apr 1.
3
Fast fluorescence holographic microscopy.
Proc SPIE Int Soc Opt Eng. 2014 Mar 12;8949. doi: 10.1117/12.2041839.
4
Spectrally resolved multidepth fluorescence imaging.
J Biomed Opt. 2011 Sep;16(9):096015. doi: 10.1117/1.3626211.
5
Quantitative phase imaging with scanning holographic microscopy: an experimental assessment.
Biomed Eng Online. 2006 Nov 28;5:63. doi: 10.1186/1475-925X-5-63.
6
Point-spread function synthesis in scanning holographic microscopy.
J Opt Soc Am A Opt Image Sci Vis. 2006 Jul;23(7):1708-17. doi: 10.1364/josaa.23.001708.
本文引用的文献
1
Two-pupil synthesis of optical transfer functions.
Appl Opt. 1978 Apr 1;17(7):1141-51. doi: 10.1364/AO.17.001141.
2
Method of two-dimensional bipolar incoherent image processing by acousto-optic two-pupil synthesis.
Opt Lett. 1985 May 1;10(5):197-9. doi: 10.1364/ol.10.000197.
3
Optical transfer function of an acousto-optic heterodyning image processor.
Opt Lett. 1979 Oct 1;4(10):317-9. doi: 10.1364/ol.4.000317.
4
A new microscopic principle.
Nature. 1948 May 15;161(4098):777. doi: 10.1038/161777a0.
5
Three-dimensional holographic fluorescence microscopy.
Opt Lett. 1997 Oct 1;22(19):1506-8. doi: 10.1364/ol.22.001506.
6
Digital holography for quantitative phase-contrast imaging.
Opt Lett. 1999 Mar 1;24(5):291-3. doi: 10.1364/ol.24.000291.
7
Point-spread function synthesis in scanning holographic microscopy.
J Opt Soc Am A Opt Image Sci Vis. 2006 Jul;23(7):1708-17. doi: 10.1364/josaa.23.001708.
8
Scanning holographic microscopy with transverse resolution exceeding the Rayleigh limit and extended depth of focus.
J Opt Soc Am A Opt Image Sci Vis. 2005 May;22(5):892-8. doi: 10.1364/josaa.22.000892.
9
A comparison of image restoration approaches applied to three-dimensional confocal and wide-field fluorescence microscopy.
J Microsc. 1999 Jan;193(1):50-61. doi: 10.1046/j.1365-2818.1999.00421.x.
10
Digital in-line holography for biological applications.
Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11301-5. doi: 10.1073/pnas.191361398.
Zotero 插件