Sinclair Michael B, Haaland David M, Timlin Jerilyn A, Jones Howland D T
Department 1824, Sandia National Laboratories. Mail Stop 1411, Albuquerque, New Mexico 87185, USA.
Appl Opt. 2006 Aug 20;45(24):6283-91. doi: 10.1364/ao.45.006283.
We have developed a new, high performance, hyperspectral microscope for biological and other applications. For each voxel within a three-dimensional specimen, the microscope simultaneously records the emission spectrum from 500 nm to 800 nm, with better than 3 nm spectral resolution. The microscope features a fully confocal design to ensure high spatial resolution and high quality optical sectioning. Optical throughput and detection efficiency are maximized through the use of a custom prism spectrometer and a backside thinned electron multiplying charge coupled device (EMCCD) array. A custom readout mode and synchronization scheme enable 512-point spectra to be recorded at a rate of 8300 spectra per second. In addition, the EMCCD readout mode eliminates curvature and keystone artifacts that often plague spectral imaging systems. The architecture of the new microscope is described in detail, and hyperspectral images from several specimens are presented.
我们已经开发出一种新型的高性能高光谱显微镜，用于生物学及其他应用。对于三维样本中的每个体素，该显微镜可同时记录从500纳米到800纳米的发射光谱，光谱分辨率优于3纳米。该显微镜采用全共焦设计，以确保高空间分辨率和高质量光学切片。通过使用定制棱镜光谱仪和背面减薄电子倍增电荷耦合器件（EMCCD）阵列，光学通量和检测效率得以最大化。一种定制的读出模式和同步方案能够以每秒8300个光谱的速率记录512点光谱。此外，EMCCD读出模式消除了经常困扰光谱成像系统的曲率和梯形失真伪像。详细描述了新型显微镜的架构，并展示了来自几个样本的高光谱图像。
