Wenz I, Loesche W, Till U, Petermann H, Horn A
J Chromatogr. 1976 May 12;120(1):187-96. doi: 10.1016/s0021-9673(01)99011-9.
The anion-exchange chromatography of commercial NADH using a potassium bicarbonate solution as eluent yields highly pure NADH with good stability. Twelve compounds are also separated which act as dehydrogenase inhibitors. The main impurities are further characterized. The compound mainly responsible for residual optical density in commercial NADH preparations is probably a stereoisomer of NADH which is in reversible equilibrium with NADH at pH values in the range 5-7. A method of thin-layer chromatography, to check commercial NADH preparations for impurities, is described.
使用碳酸氢钾溶液作为洗脱剂对市售烟酰胺腺嘌呤二核苷酸（NADH）进行阴离子交换色谱分析，可得到具有良好稳定性的高纯度NADH。同时还分离出12种作为脱氢酶抑制剂的化合物。对主要杂质进行了进一步表征。市售NADH制剂中导致残留光密度的主要化合物可能是NADH的一种立体异构体，它在pH值为5 - 7的范围内与NADH处于可逆平衡状态。本文描述了一种用于检测市售NADH制剂中杂质的薄层色谱法。
