Melton Terry, Holland Charity
Mitotyping Technologies, 2565 Park Center Boulevard, Suite 200, State College, PA 16801, USA.
J Forensic Sci. 2007 Nov;52(6):1305-7. doi: 10.1111/j.1556-4029.2007.00553.x. Epub 2007 Sep 15.
Since July 2004, Mitotyping Technologies has been amplifying and sequencing a approximately 150 base pair fragment of mitochondrial DNA (mtDNA) that codes for 12S ribosomal RNA, to identify the species origin of nonhuman casework samples. The approximately 100 base pair sequence product is searched at http://www.ncbi.nlm.nih.gov/BLAST and the species match is reported. The use of this assay has halved the number of samples for which no mtDNA results are obtained and is especially useful on hairs and degraded samples. The availability of species determination may aid forensic investigators in opening or closing off lines of inquiry where a highly probative but challenging sample has been collected.
自2004年7月以来，Mitotyping Technologies公司一直在扩增和测序一段约150个碱基对的线粒体DNA（mtDNA）片段，该片段编码12S核糖体RNA，以确定非人类法医样本的物种来源。大约100个碱基对的序列产物在http://www.ncbi.nlm.nih.gov/BLAST网站上进行搜索，并报告物种匹配情况。这种检测方法的使用使未获得mtDNA结果的样本数量减少了一半，在毛发和降解样本上尤其有用。物种鉴定的可用性可能有助于法医调查人员在收集到具有高度证明力但具有挑战性的样本时，展开或结束调查线索。
