Stsepetova Jelena, Sepp Epp, Julge Kaja, Vaughan Elaine, Mikelsaar Marika, de Vos Willem M
Department of Microbiology, Medical Faculty, University of Tartu, Tartu, Estonia.
FEMS Immunol Med Microbiol. 2007 Nov;51(2):260-9. doi: 10.1111/j.1574-695X.2007.00306.x. Epub 2007 Sep 14.
The composition of intestinal microbiota and the Bifidobacterium group community in 20 allergic and 20 nonallergic 5-year-old children was visualized by PCR-denaturing gradient gel electrophoresis (DGGE). The number of dominant bands in the DGGE profiles was smaller in allergic children than in nonallergic children (P<0.001). Allergic children mainly formed a single group upon cluster analysis, whereas nonallergic children were divided between four different groups. In allergic children the Bifidobacterium adolescentis species prevailed, and in nonallergic children the Bifidobacterium catenulatum/pseudocatenulatum prevailed (P=0.01 and P=0.01, respectively). The less diverse composition of intestinal microbiota and prevalence of particular species of Bifidobacterium were characteristic of allergic children even at the age of 5 years.
通过聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）对20名过敏和20名非过敏的5岁儿童的肠道微生物群组成和双歧杆菌菌群群落进行可视化分析。过敏儿童DGGE图谱中的优势条带数量比非过敏儿童少（P<0.001）。聚类分析显示，过敏儿童主要形成一个单一的组，而非过敏儿童则分为四个不同的组。在过敏儿童中青春双歧杆菌占优势，在非过敏儿童中链状双歧杆菌/假链状双歧杆菌占优势（分别为P=0.01和P=0.01）。即使在5岁时，肠道微生物群组成多样性较低以及特定双歧杆菌物种的流行也是过敏儿童所特有的。
