Laboratoire de Biologie EA3199, Conservatoire national des arts et métiers, 2 rue Conté, Paris, France.
Anaerobe. 2010 Aug;16(4):433-8. doi: 10.1016/j.anaerobe.2010.06.005. Epub 2010 Jun 19.
Amoxicillin is a beta-lactam antibiotic largely used in childhood. However only few studies described its impact on composition of children gut microbiota, in particular on Bifidobacterium populations considered as beneficial microorganisms. In this study, the impact on faecal Bifidobacterium species of a seven-day amoxicillin treatment was quantitatively and qualitatively assessed in infants during an episode of acute respiratory infection.
Faecal samples from 31 infants were obtained on day 0 (just before amoxicillin therapy) and on day 7 (the end of therapy). Total DNA was extracted and bifidobacteria were quantified using real-time PCR. Predominant Bifidobacterium species were then identified using specific PCR-TTGE.
Bifidobacteria concentrations were not significantly altered by amoxicillin compared to the healthy group. However, amoxicillin treatment induced a complete disappearance of Bifidobacterium adolescentis species (occurrence rate of 0% versus 36.4% in healthy group, P < 0.001), a significant decrease in the occurrence rate of Bifidobacterium bifidum (23% versus 54.5% in healthy group, P < 0.05), but did not affect Bifidobacterium longum (93.5% versus 100% in healthy group) and Bifidobacterium pseudocatenulatum/B. catenulatum (about 55% in both groups). The number of Bifidobacterium species per microbiota significantly decreased from 2.5 +/- 1 for healthy group to 1.8 +/- 0.9 for treated infants (P < 0.05).
This study showed that a 7 day amoxicillin treatment did not alter the counts of Bifidobacterium. However amoxicillin can have an impact by changing the microbiota at the species level and decreased the diversity of this population.
阿莫西林是一种常用于儿童的β-内酰胺类抗生素。然而，只有少数研究描述了它对儿童肠道微生物群组成的影响，特别是对双歧杆菌属的影响，双歧杆菌属被认为是有益的微生物。在这项研究中，在急性呼吸道感染期间，评估了 7 天阿莫西林治疗对婴儿粪便双歧杆菌属的数量和质量的影响。
31 名婴儿在第 0 天（阿莫西林治疗前）和第 7 天（治疗结束时）获得粪便样本。提取总 DNA，实时 PCR 定量双歧杆菌。然后使用特异性 PCR-TTGE 鉴定主要双歧杆菌属。
与健康组相比，阿莫西林治疗并未显著改变双歧杆菌属的浓度。然而，阿莫西林治疗导致双歧杆菌属青少年种完全消失（发生率为 0%对健康组的 36.4%，P<0.001），双歧杆菌属双歧杆菌种的发生率显著降低（23%对健康组的 54.5%，P<0.05），但不影响长双歧杆菌属和假链状双歧杆菌/双歧杆菌属（健康组分别为 93.5%和 100%）。每个微生物群落的双歧杆菌属种类数量从健康组的 2.5 +/- 1 显著减少到治疗组婴儿的 1.8 +/- 0.9（P<0.05）。
这项研究表明，7 天阿莫西林治疗不会改变双歧杆菌属的数量。然而，阿莫西林可以通过改变物种水平的微生物群产生影响，并降低该种群的多样性。
