Drell Tiina, Larionova Anneli, Voor Tiia, Simm Jaak, Julge Kaja, Heilman Kaire, Tillmann Vallo, Štšepetova Jelena, Sepp Epp
Institute of Microbiology, University of Tartu, Tartu, Estonia.
Curr Microbiol. 2015 Aug;71(2):177-83. doi: 10.1007/s00284-015-0815-9. Epub 2015 Apr 14.
Although gut microbiota has been studied relatively extensively in the context of allergic diseases, there have been several contradictions between these studies. By applying high-throughput sequencing, we aimed to analyze the differences in gut microbiota between atopic and healthy children at 5 and 12 years of age. 51 stool samples were collected from 14 atopic and 15 healthy children and analyzed with 454 pyrosequencing of the 16S rRNA gene. At the ages of 5 and 12 years, Bacteroides, Prevotella, and Dialister dominated gut microbiota in both atopic and healthy groups of children. Children in the atopic group had lower abundance and prevalence of Akkermansia in gut microbiota than their healthy counterparts. Thus, the composition of gut microbiota does not seem to be significantly different between atopic and healthy children, but lower abundance and prevalence of Akkermansia indicate that this bacterium may accompany or play a role in IgE-mediated atopic diseases.
尽管肠道微生物群在过敏性疾病背景下已得到较为广泛的研究，但这些研究之间存在一些矛盾之处。通过应用高通量测序技术，我们旨在分析5岁和12岁特应性儿童与健康儿童肠道微生物群的差异。从14名特应性儿童和15名健康儿童中收集了51份粪便样本，并对16S rRNA基因进行454焦磷酸测序分析。在5岁和12岁时，拟杆菌属、普雷沃菌属和戴利斯特菌属在特应性和健康儿童组的肠道微生物群中均占主导地位。特应性组儿童肠道微生物群中阿克曼氏菌的丰度和流行率低于健康儿童。因此，特应性儿童与健康儿童的肠道微生物群组成似乎没有显著差异，但阿克曼氏菌较低的丰度和流行率表明，这种细菌可能与IgE介导的特应性疾病相伴或发挥作用。
