Okuda Hiroaki, Miyata Shingo, Mori Yasutake, Tohyama Masaya
Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
FEBS Lett. 2007 Oct 2;581(24):4754-60. doi: 10.1016/j.febslet.2007.08.075. Epub 2007 Sep 6.
The Drosophila planar cell polarity (PCP) gene prickle has been previously indicated as one of the regulators of gastrulation in the early embryonic stage. However, the functional role of prickle in the brain in particular is not known. We first indicated that mouse Prickle1 and Prickle2 are continually expressed in the brain throughout the embryonic stages and are observed to be specifically expressed in the postmitotic neurons. Furthermore, Prickle1 or Prickle2 depletion effectively decreases the neurite outgrowth levels of mouse neuroblastoma Neuro2a cells. These results indicate that mouse Prickle1 and Prickle2 possibly regulate positive neurite formation during brain development.
果蝇平面细胞极性（PCP）基因prickle先前已被指出是早期胚胎阶段原肠胚形成的调节因子之一。然而，prickle在大脑中的具体功能作用尚不清楚。我们首先表明，小鼠Prickle1和Prickle2在整个胚胎阶段的大脑中持续表达，并观察到它们在有丝分裂后的神经元中特异性表达。此外，Prickle1或Prickle2的缺失有效地降低了小鼠神经母细胞瘤Neuro2a细胞的神经突生长水平。这些结果表明，小鼠Prickle1和Prickle2可能在大脑发育过程中调节正向神经突的形成。
