Ikegaya Hiroshi, Motani Hisako, Sakurada Koichi, Sato Keita, Akutsu Tomoko, Yoshino Mineo
National Research Institute of Police Science, Chiba 277-0882, Japan.
J Virol Methods. 2008 Jan;147(1):78-85. doi: 10.1016/j.jviromet.2007.08.010. Epub 2007 Sep 14.
Using 50 forensic blood samples, the latent membrane protein 2A (LMP-2A) gene of Epstein-Barr virus (EBV) DNA was amplified to find a geographic correlation among the EBV genotypes. EBV DNA was detected in nine samples. From a phylogenetic analysis using 18 reported sequences as a reference, six EBV subtypes (Ia, Ib, Ic IIa, IIb, and IIc) were found. Japanese isolates were included in subtypes Ia or IIa. All the Asian reference isolates, except isolate D6, were included in subtype Ia or IIa. Mediterranean, an Alaskan and other African isolates were included in types Ib, Ic, IIb and IIc. The EBV genotype in the LMP-2A gene was thus demonstrated as being correlated with the host's geographical location. Typing in the EBV-associated nuclear antigen 2 gene was not related to that in the LMP-2A gene. Detection of the EBV genotype in the LMP-2A gene may be useful for determining the geographical origins of unidentified cadavers.
利用50份法医血液样本，对爱泼斯坦-巴尔病毒（EBV）DNA的潜伏膜蛋白2A（LMP-2A）基因进行扩增，以寻找EBV基因型之间的地理相关性。在9份样本中检测到EBV DNA。以18条已报道序列为参考进行系统发育分析，发现了6种EBV亚型（Ia、Ib、Ic、IIa、IIb和IIc）。日本分离株属于Ia或IIa亚型。除分离株D6外，所有亚洲参考分离株均属于Ia或IIa亚型。地中海地区、阿拉斯加和其他非洲分离株属于Ib、Ic、IIb和IIc型。因此，LMP-2A基因中的EBV基因型与宿主的地理位置相关。EBV相关核抗原2基因的分型与LMP-2A基因的分型无关。检测LMP-2A基因中的EBV基因型可能有助于确定身份不明尸体的地理来源。
