Virology Department,University of Sydney, Westmead, Australia. mlay4697 @ uni.sydney.edu.au
Intervirology. 2012;55(5):372-9. doi: 10.1159/000334693. Epub 2012 Jan 24.
To identify Epstein-Barr virus (EBV) genotypes and strains in samples from individuals with and without a first diagnosis of central nervous system (CNS) demyelinating disease (a possible precursor to multiple sclerosis) and patients with EBV-associated diseases in Australia.
Samples from 55 EBV DNA and serology positive subjects including individuals with (n = 17) and without (n = 21) a first clinical diagnosis of CNS demyelination and patients with EBV-related diseases (n = 17) were examined. EBV genotype and strain were identified by sequence mutations within the Epstein-Barr nuclear antigen-2 region (EBNA-2) using DNA sequence analysis.
Both EBV genotypes, A and B, were detected (genotype A, 54/55, 98.2%; genotype B, 1/55, 1.8%). Within genotype A, GD1 was the most commonly detected strain (42/54, 77.7%), followed by B95-8 (9/54, 16.7%) and M-ABA (3/54, 5.6%). Genotype B, strain AG876, was found in one individual with CNS demyelinating disease.
EBV genotype A and the GD1 strain were the common EBV genotypes isolated from individuals with and without CNS demyelinating disease, and in subjects with various EBV-related diseases. Although disease-specific genotypes or strains were not identified, this study provides useful insights into the molecular epidemiology of EBV infection in Australia.
鉴定澳大利亚首例中枢神经系统（CNS）脱髓鞘疾病（多发性硬化症的可能前兆）患者和 EBV 相关疾病患者与无该病患者的 EBV 基因型和毒株。
对 55 例 EBV DNA 和血清学阳性样本中的个体进行了检测，包括有（n=17）和无（n=21）首次 CNS 脱髓鞘临床诊断的个体，以及 EBV 相关疾病患者（n=17）。通过 DNA 序列分析，在 Epstein-Barr 核抗原-2 区（EBNA-2）的序列突变中鉴定 EBV 基因型和毒株。
均检测到 EBV 基因型 A 和 B（基因型 A，54/55，98.2%；基因型 B，1/55，1.8%）。在基因型 A 中，GD1 是最常见的毒株（42/54，77.7%），其次是 B95-8（9/54，16.7%）和 M-ABA（3/54，5.6%）。基因型 B，株 AG876，在一例 CNS 脱髓鞘疾病患者中发现。
基因型 A 和 GD1 株是从有和无 CNS 脱髓鞘疾病患者以及 EBV 相关疾病患者中分离出的常见 EBV 基因型。尽管未鉴定出特定疾病的基因型或毒株，但本研究为 EBV 感染的分子流行病学在澳大利亚的研究提供了有用的见解。
