Uchida Tomoyuki, Ikeda Seiichi, Oura Hiroyuki, Tada Mika, Nakano Takuma, Fukuda Toshio, Matsuda Takehisa, Negoro Makoto, Arai Fumihito
Department of Micro-Nano Systems Engineering, Graduate School of Engineering, Nagoya University, Japan.
J Biotechnol. 2008 Jan 20;133(2):213-8. doi: 10.1016/j.jbiotec.2007.08.017. Epub 2007 Aug 14.
Biodegradable scaffolds are of great value in tissue engineering. We have developed a method for fabricating patient-specific vascular scaffolds from a biocompatible and biodegradable polymer, poly(L-lactide-co-epsilon-caprolactone). This method's usefulness is due to flexibility in the choice of materials and vascular configurations. Here, we present a way to fabricate scaffolds of human carotid artery by combining processes of rapid prototyping, lost wax, dip coating, selective dissolution, and salt leaching. The result was the successful development of porous biodegradable scaffolds, with mechanical strength covering the range of human blood vessels (1-3 MPa). Human umbilical vein endothelial cells were also cultured on the scaffolds and their biocompatibility was confirmed by cell growth. The Young's modulus of scaffolds could be controlled by changing polymer concentration and porosity. The wall thickness of the tubular scaffold was also controllable by adjusting polymer concentration and pull-up velocity during dip coating. We believe that this fabrication technique can be applied to patient-specific regeneration of blood vessels.
可生物降解支架在组织工程中具有重要价值。我们已经开发出一种方法，可利用生物相容性和可生物降解的聚合物聚（L-丙交酯-共-ε-己内酯）制造针对患者的血管支架。该方法的实用性在于材料选择和血管结构方面的灵活性。在此，我们展示一种通过结合快速成型、失蜡法、浸涂、选择性溶解和盐析工艺来制造人颈动脉支架的方法。结果成功开发出了多孔可生物降解支架，其机械强度涵盖人体血管的范围（1-3兆帕）。人脐静脉内皮细胞也在支架上进行了培养，并且通过细胞生长证实了它们的生物相容性。支架的杨氏模量可通过改变聚合物浓度和孔隙率来控制。管状支架的壁厚也可通过在浸涂过程中调整聚合物浓度和提拉速度来控制。我们相信这种制造技术可应用于针对患者的血管再生。
