Department of Chemical Engineering and Biotechnology, University of Sherbrooke, 2500 University Boulevard, Sherbrooke, Québec, Canada J1K 2R1.
J Biomed Mater Res A. 2010 Jun 1;93(3):1140-50. doi: 10.1002/jbm.a.32608.
In spite of the present advances in the scaffolds fabrication and bioreactor systems, the ability to create functional thick tissue masses in vitro is still a great tissue engineering challenge. To overcome this problem, the fabrication of a capillary bed, for nutrient supply to and waste product removal from the tissue engineering construct as it grows, is essential. However, the technical construction of a capillary-like architecture is complex and challenging. This study reports, for the very first time, a simple method to design and fabricate a porous hollow membrane sheet (PHMsh) to provide both a capillary bed and a scaffold to support tissue growth. The PHMsh composed of a flexible porous sheet involving parallel porous channels and can be used as flat-, rolled-, or sandwiched-shape scaffold. The PHMsh was fabricated from poly(epsilon-caprolactone) polymer solution using solvent casting methods (i.e., immersion precipitation and air casting). Optical microscopy and scanning electron microscopy were used for the morphological analyses. The PHMsh was surface treated using n-hepthylamine plasma polymer (HApp) and X-ray photoelectron spectroscopy confirmed successful surface coating. Human umbilical vein endothelial cells (HUVECs) and fibroblast cells were used to evaluate the capability of PHMsh toward cell adhesion. The HApp coating enhanced both HUVEC and fibroblast cells adhesion. The obtained preliminary results demonstrated the successful fabrication of the PHMsh, with potential application for tissue engineering scaffolds, particularly in large tissue mass generation under perfusion systems in vitro, which is our future research direction.
尽管目前在支架制造和生物反应器系统方面取得了进展，但在体外创造功能性厚组织块的能力仍然是一个巨大的组织工程挑战。为了克服这个问题，制造一个毛细血管床对于营养物质的供应和组织工程构建体在生长过程中废物的去除是至关重要的。然而，毛细血管样结构的技术构建是复杂和具有挑战性的。本研究首次报道了一种简单的方法来设计和制造多孔空心膜片（PHMsh），以提供毛细血管床和支持组织生长的支架。PHMsh 由涉及平行多孔通道的柔性多孔片组成，可以用作平板、卷板或夹层形状的支架。PHMsh 是使用溶剂铸造方法（即浸没沉淀和空气铸造）从聚己内酯聚合物溶液中制造的。使用光学显微镜和扫描电子显微镜进行形态分析。通过正庚胺等离子体聚合物（HApp）对 PHMsh 进行表面处理，X 射线光电子能谱证实了成功的表面涂层。人脐静脉内皮细胞（HUVEC）和成纤维细胞用于评估 PHMsh 对细胞黏附的能力。HApp 涂层增强了 HUVEC 和成纤维细胞的黏附。初步结果表明成功制造了 PHMsh，具有组织工程支架的潜在应用，特别是在体外灌注系统下生成大组织块，这是我们未来的研究方向。
