Sahoo Sambit, Cho-Hong James Goh, Siew-Lok Toh
Tissue Repair Lab, Division of Bioengineering, National University of Singapore, Singapore.
Biomed Mater. 2007 Sep;2(3):169-73. doi: 10.1088/1748-6041/2/3/001. Epub 2007 Aug 23.
Fibre-based scaffolds have been widely used for tendon and ligament tissue engineering. Knitted scaffolds have been proved to favour collagenous matrix deposition which is crucial for tendon/ligament reconstruction. However, such scaffolds have the limitation of being dependent on a gel system for cell seeding, which is unstable in a dynamic environment such as the knee joint. This study developed three types of hybrid scaffolds, based on knitted biodegradable polyester scaffolds, aiming to improve mechanical properties and cell attachment and proliferation on the scaffolds. The hybrid scaffolds were created by coating the knitted scaffolds with a thin film of poly (epsilon-caprolactone) (group I), poly (D, L-lactide-co-glycolide) nanofibres (group II) and type 1 collagen (group III). Woven scaffolds were also fabricated and compared with the various hybrid scaffolds in terms of their mechanical properties during in vitro degradation and cell attachment and growth. This study demonstrated that the coating techniques could modulate the mechanical properties and facilitate cell attachment and proliferation in the hybrid scaffold, which could be applied with promise in tissue engineering of tendons/ligaments.
基于纤维的支架已广泛应用于肌腱和韧带组织工程。编织支架已被证明有利于胶原基质沉积，这对肌腱/韧带重建至关重要。然而，这种支架存在依赖凝胶系统进行细胞接种的局限性，在诸如膝关节等动态环境中不稳定。本研究基于编织的可生物降解聚酯支架开发了三种类型的混合支架，旨在改善支架的力学性能以及细胞附着和增殖。混合支架是通过用聚（ε-己内酯）薄膜（I组）、聚（D，L-丙交酯-共-乙交酯）纳米纤维（II组）和I型胶原（III组）涂覆编织支架而制成的。还制备了编织支架，并在体外降解过程中的力学性能以及细胞附着和生长方面与各种混合支架进行了比较。本研究表明，涂层技术可以调节混合支架的力学性能并促进细胞附着和增殖，有望应用于肌腱/韧带的组织工程。
