Kiszel Petra, Makó Veronika, Prohászka Zoltán, Cervenak László
3rd Department of Internal Medicine, Semmelweis University, Budapest, Hungary.
Cytokine. 2007 Oct;40(1):17-22. doi: 10.1016/j.cyto.2007.08.001. Epub 2007 Sep 14.
The IL-6 is a typical pleiotropic cytokine, which regulates T cell response, B cell differentiation and immunoglobulin production. Endothelial cells can produce large amounts of IL-6. SNP at position -174 (G/C) in the IL-6 promoter region was found to be associated with a series of complex diseases. In this study we analyzed whether IL-6 -174 G/C polymorphism has any effect on IL-6 production of in vitro cultured HUVECs. Thirty-three fresh umbilical cords were recruited from healthy pregnancies. The endothelial cells isolated from human umbilical cords were genotyped for IL-6 -174 SNP. C allele frequency was 0.379. The IL-6 production of each primary HUVEC line was measured after IL-1beta or LPS treatment by ELISA. Serial dilutions of the stimulating agents were applied and maximum amount of produced IL-6 (R(max)) and stimulator concentrations at half-maximal IL-6 response (MR(50)) were calculated for each of the cell lines. IL-6 production was not associated with IL-6 -174 SNP genotypes or with presence of C allele. Our results showed that IL-6 production of HUVEC after proinflammatory stimulation was not influenced by IL-6 -174 SNP. Further functional studies are required to compare differences and similarities in IL-6 -174 SNP dependent expression of IL-6 among various cell types.
白细胞介素-6是一种典型的多效性细胞因子，可调节T细胞反应、B细胞分化和免疫球蛋白的产生。内皮细胞可产生大量白细胞介素-6。白细胞介素-6启动子区域-174位（G/C）的单核苷酸多态性（SNP）被发现与一系列复杂疾病相关。在本研究中，我们分析了白细胞介素-6 -174 G/C多态性是否对体外培养的人脐静脉内皮细胞（HUVECs）产生白细胞介素-6有任何影响。从健康孕妇中采集了33根新鲜脐带。对从人脐带中分离的内皮细胞进行白细胞介素-6 -174 SNP基因分型。C等位基因频率为0.379。用酶联免疫吸附测定法（ELISA）检测白细胞介素-1β或脂多糖（LPS）处理后各原代HUVEC系的白细胞介素-6产生情况。应用刺激剂的系列稀释液，并计算每个细胞系产生的白细胞介素-6的最大量（R(max)）和白细胞介素-6反应达到半数最大值时的刺激剂浓度（MR(50)）。白细胞介素-6的产生与白细胞介素-6 -174 SNP基因型或C等位基因的存在无关。我们的结果表明，促炎刺激后HUVEC产生白细胞介素-6不受白细胞介素-6 -174 SNP的影响。需要进一步的功能研究来比较不同细胞类型中白细胞介素-6 -174 SNP依赖性白细胞介素-6表达的异同。
