Keck C, Herchenbach D, Pfisterer J, Breckwoldt M
Dept. of Obstetrics and Gynecology at the University of Freiburg, Germany.
Exp Clin Endocrinol Diabetes. 1998;106(4):334-9. doi: 10.1055/s-0029-1211994.
The present investigation was performed to study the effects of steroids on interleukin-6 (IL-6) production and on proliferation of human umbilical vein endothelial cells (HUVEC). HUVEC were isolated and cultured in 24-well dishes until confluency was achieved. Afterwards cells were stimulated with either 17beta-estradiol or progesterone at concentrations of 10(-12)-10(-6) mol/l. IL-6 concentrations in cell supernatants were measured by ELISA and cell proliferation was determined by flow-cytometric assessment of S-phase-cells. 17Beta-estradiol significantly inhibited basal IL-6 secretion at doses of 10(-12)-10(-6) mol/l whereas progesterone had no measurable effects. Neither 17beta-estradiol nor progesterone affected the proliferation rate of endothelial cells. The results of our study suggest that 17beta-estradiol at non-proliferative doses regulates IL-6 secretion of endothelial cells and thereby modulates processes of vascular physiology.
本研究旨在探讨类固醇对人脐静脉内皮细胞（HUVEC）白细胞介素-6（IL-6）产生及细胞增殖的影响。分离HUVEC并接种于24孔板中培养至汇合。之后，用浓度为10（-12）-10（-6）mol/l的17β-雌二醇或孕酮刺激细胞。通过ELISA法检测细胞上清液中IL-6浓度，通过流式细胞术评估S期细胞来测定细胞增殖。17β-雌二醇在10（-12）-10（-6）mol/l剂量时可显著抑制基础IL-6分泌，而孕酮无明显作用。17β-雌二醇和孕酮均不影响内皮细胞的增殖率。我们的研究结果表明，非增殖剂量的17β-雌二醇可调节内皮细胞IL-6分泌，从而调节血管生理过程。
