Ikeda Kayo, Morikawa Norifumi, Kuribayashi Misa, Ikawa Kazuro, Nomura Kenichi, Taniwaki Masafumi
Department of Clinical Pharmacotherapy, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan.
J Pharm Biomed Anal. 2007 Dec 21;45(5):811-6. doi: 10.1016/j.jpba.2007.08.004. Epub 2007 Aug 10.
A simple, rapid, and precise HPLC method using ultrafiltration to remove plasma protein was developed to determine cefozopran concentrations in human plasma for real-time therapeutic drug monitoring. Plasma was separated by centrifugation at 4 degrees C from blood collected in heparinized vacuum tubes. Cefozopran and an internal standard were detected by ultraviolet absorbances at 235 nm with no interfering plasma peak. The calibration curve of cefozopran in human plasma was linear from 0.2 to 200 microg/ml. The limit of detection was 0.05 microg/ml. The assay was applied to febrile neutropenia patients in a clinical setting.
