Rieck W, Platt D
Institute of Gerontology-Chair of Internal Medicine, University of Erlangen-Nuremberg, Germany.
Clin Lab. 2000;46(9-10):477-82.
In the present study we developed a new sensitive and selective HPLC-assay for the determination of cefditoren (ME 1206) in the plasma. The column switching technique was used to regenerate the pre-column and the analytical column separately. This method is suitable for pharmacokinetic studies and for drug monitoring. Thus, we measured plasma samples of 15 elderly patients (age range: 67-88 years), receiving cefditoren-pivoxil therapy because of infections of the respiratory tract. In addition, these patients were treated with several co-medications because of further diseases. With our HPLC-method no interfering peaks could be detected at the retention time of cefditoren (10.67 min) or of the internal standard (16.62 min). The lowest plasma concentration of cefditoren to be quantified was 25 ng/ml (R.S.D.= 15.5%, n = 5).
在本研究中，我们开发了一种新的灵敏且具选择性的高效液相色谱法（HPLC），用于测定血浆中的头孢妥仑（ME 1206）。采用柱切换技术分别对预柱和分析柱进行再生。该方法适用于药代动力学研究和药物监测。因此，我们测定了15例老年患者（年龄范围：67 - 88岁）的血浆样本，这些患者因呼吸道感染接受头孢妥仑匹酯治疗。此外，由于患有其他疾病，这些患者还同时接受了几种联合用药治疗。使用我们的HPLC方法，在头孢妥仑（保留时间10.67分钟）或内标物（保留时间16.62分钟）的保留时间处未检测到干扰峰。可定量的头孢妥仑最低血浆浓度为25 ng/ml（相对标准偏差= 15.5%，n = 5）。
